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. 2015 Jun 1;8(6):6870-80.
eCollection 2015.

rhEPO affects apoptosis in hippocampus of aging rats by upregulating SIRT1

Haiqin Wu  1 , Huqing Wang  1 , Wenting Zhang  1 , Xuanhui Wei  1 , Jiaxin Zhao  1 , Pu Yan  1 , Chao Liu  1
Affiliations

rhEPO affects apoptosis in hippocampus of aging rats by upregulating SIRT1

Haiqin Wu et al. Int J Clin Exp Pathol. .

Abstract

The aim of this study was to elucidate the signaling pathway involved in the anti-aging effect of erythropoietin (EPO) and to clarify whether recombinant human EPO (rhEPO) affects apoptosis in the aging rat hippocampus by upregulating Sirtuin 1 (SIRT1). In this study, a rat model of aging was established using D-galactose. Behavioral changes were monitored by the Morris water maze test. Using immunohistochemistry, we studied the expression of SIRT1, B-cell lymphoma/leukemia-2 gene (Bcl-2), and Bcl-2 associated X protein (Bax) expression, and apoptotic cells in the hippocampus of a rat model of aging in which rhEPO was intraperitoneally injected. The escape latency in rats from the EPO group shortened significantly; however, the number of platform passes increased significantly from that in the D-gal group (P < 0.05). Compared to the D-gal group, in the EPO group, the number of SIRT1 and Bcl-2-positive cells increased (P < 0.05), but the number of Bax-positive cells and apoptotic cells decreased in the hippocampus of aging rats (P < 0.05). These results suggest that rhEPO regulates apoptosis-related genes and affects apoptosis in the hippocampus of aging rats by upregulating SIRT. This may be one of the important pathways underlying the anti-aging property of EPO.

Keywords: B-cell lymphoma/leukemia-2 gene; Bcl-2 association X protein; Erythropoietin; Sirtuin 1; aging.

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Figures

Figure 1
Figure 1
The mean weight of each group of rats before and after model-making.
Figure 2
Figure 2
Pyramidal neurons of hippocampal CA1 in different group. (Nissl staining, optical microscope, ×400). A: Sham group; B: D-gal group; C: EPO group; D: VitE group.
Figure 3
Figure 3
Expression of SIRT1, Bcl-2, Bax and apoptosis in CA1 region each group (immunohistochemistry, TUNEL staining, ×400). A: Sham group; B: D-gal group; C: EPO group; D: VitE group.
See this image and copyright information in PMC

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