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. 2015 Jul 30;16(8):17469-81.
doi: 10.3390/ijms160817469.

Interleukin-17A Gene Expression in Morbidly Obese Women

Fernando Zapata-Gonzalez  1 , Teresa Auguet  2   3 , Gemma Aragonès  4 , Esther Guiu-Jurado  5 , Alba Berlanga  6 , Salomé Martinez  7 , Andreu Martí  8 , Fátima Sabench  9 , Mercé Hernandez  10 , Carmen Aguilar  11 , Joan Josep Sirvent  12 , Rosa Jorba  13 , Daniel Del Castillo  14 , Cristóbal Richart  15   16
Affiliations

Interleukin-17A Gene Expression in Morbidly Obese Women

Fernando Zapata-Gonzalez et al. Int J Mol Sci. .

Abstract

Data from recent studies conducted in rodent models and humans suggest that interleukin-17A (IL-17A) plays a role in the induction of inflammation in adipose tissue during obesity. The aim of this study was to assess the gene expression of IL-17A in adipose tissue of morbidly obese patients. We used RT-PCR to evaluate the expression of IL-17A and several adipo/cytokines in the visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) of 10 normal-weight control women (BMI < 25 kg/m2) and 30 morbidly obese women (MO, BMI > 40 kg/m2). We measured serum levels of IL-17A and adipo/cytokines in MO and normal weight women. IL-17A expression was significantly higher in VAT than in SAT in MO patients (p = 0.0127). It was very low in normal-weight controls in both VAT and SAT tissues. We found positive correlations between IL-17A and IL-6, lipocalin-2 and resistin in VAT of MO patients. The circulating level of IL-17A was higher in the normal-weight group than the MO patients (p = 0.032), and it was significantly related to adiponectin and TNFRII levels. In conclusion, IL-17A expression in VAT is increased in morbidly obese women, which suggests a link between obesity and innate immunity in low-grade chronic inflammation in morbidly obese women.

Keywords: IL-17A; adipo/cytokine; morbid obesity; visceral adipose tissue.

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Figures

Figure 1
Figure 1
IL-17A mRNA expression in visceral and subcutaneous adipose tissues in normal-weight subjects (A) and morbidly obese women (B). AU: arbitrary units × 104; VAT: visceral adipose tissue; SAT: subcutaneous adipose tissue. Student’s t-test was used to compare the gene expression in the two groups. Results are shown as the mean ± SD. p < 0.05 is considered statistically significant.
Figure 2
Figure 2
Correlation between mRNA expression of IL-17A and IL-6 (A), lipocalin-2 (B) and resistin (C) in visceral adipose tissue of morbidly obese women. The strength of association between variables was calculated using Spearman’s r correlation test. p < 0.05 is considered statistically significant.
Figure 3
Figure 3
Circulating levels of IL-17A in normal-weight subjects (BMI < 25) and morbidly obese women (BMI > 40). Student’s t-test was used to compare the gene expression in the two groups. Results are shown as the mean ± SD. p < 0.05 are considered statistically significant.
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