Performance of the CLSI Carba NP and the Rosco Carb Screen Assays Using North American Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa Isolates
- PMID: 26269624
- PMCID: PMC4572554
- DOI: 10.1128/JCM.01547-15
Performance of the CLSI Carba NP and the Rosco Carb Screen Assays Using North American Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa Isolates
Abstract
This study compared the performance of the Carba NP assay, published by the Clinical and Laboratory Standards Institute, and the Rosco Rapid Carb Screen kit. Carba NP had superior sensitivity, but both assays required an increased inoculum to detect carbapenemase production in isolates with blaNDM, blaIMP, and blaOXA-48.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
References
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- Centers for Disease Control and Prevention. 2012. 2012 CRE toolkit—guidance for control of carbapenem-resistant Enterobacteriaceae (CRE). Centers for Disease Control and Prevention, Atlanta, GA: http://www.cdc.gov/hai/pdfs/cre/CRE-guidance-508.pdf Accessed 8 April 2015.
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- Vasoo S, Cunningham SA, Kohner PC, Simner PJ, Mandrekar JN, Lolans K, Hayden MK, Patel R. 2013. Comparison of a novel, rapid chromogenic biochemical assay, the Carba NP Test, with the modified Hodge test for detection of carbapenemase-producing Gram-negative bacilli. J Clin Microbiol 51:3097–3101. doi:10.1128/JCM.00965-13. - DOI - PMC - PubMed
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- CLSI. 2015. Performance standards for antimicrobial susceptibility testing; twenty-fourth informational supplement. CLSI document M100-S25. Clinical and Laboratory Standards Institute, Wayne, PA.
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