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. 2015 Aug 18;10(8):e0136052.
doi: 10.1371/journal.pone.0136052. eCollection 2015.

Extended-Spectrum β-Lactamases and/or Carbapenemases-Producing Enterobacteriaceae Isolated from Retail Chicken Meat in Zagazig, Egypt

H M Abdallah  1 , E A Reuland  1 , B B Wintermans  1 , N Al Naiemi  2 , A Koek  1 , A M Abdelwahab  3 , A M Ammar  3 , A A Mohamed  3 , C M J E Vandenbroucke-Grauls  1
Affiliations

Extended-Spectrum β-Lactamases and/or Carbapenemases-Producing Enterobacteriaceae Isolated from Retail Chicken Meat in Zagazig, Egypt

H M Abdallah et al. PLoS One. .

Abstract

Objectives: The aim of the present study was to determine the prevalence and to characterize extended-spectrum β-lactamases- and/or carbapenemases-producing Enterobacteriaceae among Enterobacteriaceae isolated from retail chicken meat in Zagazig, Egypt.

Methods: One hundred and six Enterobacteriaceae isolates were collected from retail chicken meat samples purchased in Zagazig, Egypt in 2013. Species identification was done by MALDI-TOF MS. Screening for ESBL-E was performed by inoculation of isolates recovered from meat samples onto the EbSA (Cepheid Benelux, Apeldoorn, the Netherlands) selective screening agar. ESBL production was confirmed by combination disc diffusion test with clavulanic acid (Rosco, Taastrup, Denmark). Carbapenemases production was confirmed with double disk synergy tests. Resistance genes were characterized by PCR with specific primers for TEM, SHV, and CTX-M and carbapenemases (KPC, NDM, OXA-48, IMP and VIM). PCR products of CTX-M genes were purified and sequenced. Phylogenetic grouping of E. coli was performed by a PCR-based method.

Results: Of these 106 isolates 69 (65.09%) were ESBL producers. Twelve (11.32%) of these isolates were also phenotypically class B carbapenemases producer. TEM genes were detected in 61 (57.55%) isolates. 49 (46.23%) isolates harbored CTX-M genes, and 25 (23.58%) carried genes of the SHV family. All CPE belonged to the NDM group. The predominant CTX-M sequence type was CTX-M-15 (89.80%). The majority (80%) of the ESBL-EC belonged to low virulence phylogroups A and B1.

Conclusions: This is the first study from Egypt reporting high rates of ESBLs and carbapenemases (65.09% and 11.32%, respectively) in Enterobacteriaceae isolated from retail chicken meat. These results raise serious concerns about public health and food safety as retail meat could serve as a reservoir for these resistant bacteria which could be transferred to humans through the food chain.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

References

    1. Coleman K. Diazabicyclooctanes (DBOs): a potent new class of non-β-lactam β-lactamase inhibitors. Curr Opin Microbiol. 2011;14: 550–5. 10.1016/j.mib.2011.07.026 - DOI - PubMed
    1. Hughes LA, Pinchbeck G, Callaby R, Dawson S, Clegg P, Williams N. Antimicrobial prescribing practice in UK equine veterinary practice. Equine Vet J. 2013;45: 141–7. 10.1111/j.2042-3306.2012.00602.x - DOI - PubMed
    1. Allen HK, Donato J, Wang HH, Cloud-Hansen KA, Davies J, Handelsman J. Call of the wild: antibiotic resistance genes in natural environments. Nat Rev Microbiol. Nature Publishing Group; 2010;8: 251–9. 10.1038/nrmicro2312 - DOI - PubMed
    1. Essack SY. The Development of β-Lactam Antibiotics in Response to the Evolution of β-Lactamases. Pharm Res. Kluwer Academic Publishers-Plenum Publishers; 2001;18: 1391–1399. 10.1023/A:1012272403776 - DOI - PubMed
    1. Poole K. Resistance to beta-lactam antibiotics. Cell Mol Life Sci. 2004;61: 2200–23. 10.1007/s00018-004-4060-9 - DOI - PMC - PubMed

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