High-throughput pairing of T cell receptor α and β sequences
- PMID: 26290413
- DOI: 10.1126/scitranslmed.aac5624
High-throughput pairing of T cell receptor α and β sequences
Erratum in
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Erratum for the Research Article: "High-throughput pairing of T cell receptor α and β sequences" by B. Howie, A. M. Sherwood, A. D. Berkebile, J. Berka, R. O. Emerson, D. W. Williamson, I. Kirsch, M. Vignali, M. J. Rieder, C. S. Carlson, H. S. Robins.Sci Transl Med. 2015 Oct 14;7(309):309er7. doi: 10.1126/scitranslmed.aad5480. Sci Transl Med. 2015. PMID: 26468322 No abstract available.
Abstract
The T cell receptor (TCR) protein is a heterodimer composed of an α chain and a β chain. TCR genes undergo somatic DNA rearrangements to generate the diversity of T cell binding specificities needed for effective immunity. Recently, high-throughput immunosequencing methods have been developed to profile the TCR α (TCRA) and TCR β (TCRB) repertoires. However, these methods cannot determine which TCRA and TCRB chains combine to form a specific TCR, which is essential for many functional and therapeutic applications. We describe and validate a method called pairSEQ, which can leverage the diversity of TCR sequences to accurately pair hundreds of thousands of TCRA and TCRB sequences in a single experiment. Our TCR pairing method uses standard laboratory consumables and equipment without the need for single-cell technologies. We show that pairSEQ can be applied to T cells from both blood and solid tissues, such as tumors.
Copyright © 2015, American Association for the Advancement of Science.
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