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Comparative Study
. 2015 Oct;22(10):1130-2.
doi: 10.1128/CVI.00390-15. Epub 2015 Aug 19.

Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology

Hugh W F Kingston  1 , Stuart D Blacksell  2 , Ampai Tanganuchitcharnchai  3 , Achara Laongnualpanich  4 , Buddha Basnyat  5 , Nicholas P J Day  2 , Daniel H Paris  6
Affiliations
Comparative Study

Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology

Hugh W F Kingston et al. Clin Vaccine Immunol. 2015 Oct.

Abstract

This study investigated the comparative accuracy of a recombinant 56-kDa type-specific antigen-based rapid diagnostic test (RDT) for scrub typhus for the detection of IgM antibodies by using conventional serology in well-characterized serum samples from undifferentiated febrile illness patients. The RDT showed high specificity and promising comparative accuracy, with 82% sensitivity and 98% specificity for samples defined positive at an IgM indirect immunofluorescence assay positivity cutoff titer of ≥1:1,600 versus 92% and 95% at ≥1:6,400, respectively.

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Figures

FIG 1
FIG 1
Relationship between IFA titers and RDT positivity. Shown are the proportions of positive RDTs at different IFA titers plotted on a logarithmic scale. Logistic regression was used to describe the sigmoidal relationship between RDT positivity and IFA titers, illustrating the low proportions of RDT positivity at IFA titers of <1:3,200. No significant difference between the two RDT versions was observed (secondary PAb detection, red; secondary MAb detection, blue).
See this image and copyright information in PMC

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