Molecular Approaches to Identify Helicobacter pylori Antimicrobial Resistance

Abstract

Antimicrobial susceptibility testing is needed to adapt Helicobacter pylori treatment to obtain the best results. Beside the standard phenotypic methods, molecular methods are increasingly used. The value of these molecular tests is that they are quick, independent of the transport conditions, easy to standardize, and commercial kits are available. In this article, these methods are reviewed, focusing on the determination of H pylori resistance to macrolides and fluoroquinolones, and mentioning also the methods used for tetracycline and rifampin.

Keywords: Dual priming oligonucleotide; Fluorescent in situ hybridization; Fluoroquinolones; Macrolides; Polymerase chain reaction; Real-time polymerase chain reaction; Rifampins; Tetracyclines.