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Comment
. 2015 Aug 28;349(6251):936.
doi: 10.1126/science.aaa5594. Epub 2015 Aug 27.

FUNGAL BIOGEOGRAPHY. Response to Comment on "Global diversity and geography of soil fungi"

Leho Tedersoo  1 , Mohammad Bahram  2 , Sergei Põlme  3 , Sten Anslan  4 , Taavi Riit  4 , Urmas Kõljalg  4 , R Henrik Nilsson  5 , Falk Hildebrand  6 , Kessy Abarenkov  3
Affiliations
Comment

FUNGAL BIOGEOGRAPHY. Response to Comment on "Global diversity and geography of soil fungi"

Leho Tedersoo et al. Science. .

Abstract

Schadt and Rosling (Technical Comment, 26 June 2015, p. 1438) argue that primer-template mismatches neglected the fungal class Archaeorhizomycetes in a global soil survey. Amplicon-based metabarcoding of nine barcode-primer pair combinations and polymerase chain reaction (PCR)-free shotgun metagenomics revealed that barcode and primer choice and PCR bias drive the diversity and composition of microorganisms in general, but the Archaeorhizomycetes were little affected in the global study. We urge that careful choice of DNA markers and primers is essential for ecological studies using high-throughput sequencing for identification.

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Comment on

  • Fungal biogeography. Global diversity and geography of soil fungi.
    Tedersoo L, Bahram M, Põlme S, Kõljalg U, Yorou NS, Wijesundera R, Villarreal Ruiz L, Vasco-Palacios AM, Thu PQ, Suija A, Smith ME, Sharp C, Saluveer E, Saitta A, Rosas M, Riit T, Ratkowsky D, Pritsch K, Põldmaa K, Piepenbring M, Phosri C, Peterson M, Parts K, Pärtel K, Otsing E, Nouhra E, Njouonkou AL, Nilsson RH, Morgado LN, Mayor J, May TW, Majuakim L, Lodge DJ, Lee SS, Larsson KH, Kohout P, Hosaka K, Hiiesalu I, Henkel TW, Harend H, Guo LD, Greslebin A, Grelet G, Geml J, Gates G, Dunstan W, Dunk C, Drenkhan R, Dearnaley J, De Kesel A, Dang T, Chen X, Buegger F, Brearley FQ, Bonito G, Anslan S, Abell S, Abarenkov K. Tedersoo L, et al. Science. 2014 Nov 28;346(6213):1256688. doi: 10.1126/science.1256688. Science. 2014. PMID: 25430773
  • FUNGAL BIOGEOGRAPHY. Comment on "Global diversity and geography of soil fungi".
    Schadt CW, Rosling A. Schadt CW, et al. Science. 2015 Jun 26;348(6242):1438. doi: 10.1126/science.aaa4269. Science. 2015. PMID: 26113712

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