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. 2013:2013:960125.
doi: 10.1155/2013/960125. Epub 2012 Oct 1.

Effect of Quercetin on Haematobiochemical and Histological Changes in the Liver of Polychlorined Biphenyls-Induced Adult Male Wistar Rats

Kandaswamy Selvakumar  1 , Senthamilselvan Bavithra  1 , Sekaran Suganya  1 , Firdous Ahmad Bhat  1 , Gunasekaran Krishnamoorthy  2 , Jagadeesan Arunakaran  1
Affiliations

Effect of Quercetin on Haematobiochemical and Histological Changes in the Liver of Polychlorined Biphenyls-Induced Adult Male Wistar Rats

Kandaswamy Selvakumar et al. J Biomark. 2013.

Abstract

Polychlorinated biphenyls exposure damages the rat liver cells. Hematological parameters such as hemoglobin, packed cell volume, red-blood cells, white-blood cells, neutrophils, platelet counts, and RBC indices were significantly decreased. Polymorphs, eosinophil counts, and erythrocyte sedimentation rate were significantly increased. Serum liver enzymes such as aspartate transaminase, alanine transaminase, alkaline phosphatase, and gamma-glutamyl transferase were increased by PCBs treatment. Serum lipid profiles such as cholesterol, triglycerides, low-density lipoproteins and very-low-density lipoproteins were increased in PCBs-treated rats. High-density lipoprotein, total protein, albumin, globulin levels, and albumin/globulin ratio were also decreased after PCB exposure. Then levels of sodium, potassium, chloride, and bicarbonate were also altered. Serum glucose levels were increased along with total bilirubin after PCBs exposure. Simultaneous quercetin supplementation significantly protected the PCBs-induced changes of hematobiochemical parameters. Thus, quercetin shows a protective role against PCBs-induced alterations in the hematological and biochemical parameters.

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Figures

Figure 1
Figure 1
Effect of quercetin on body weight of PCBs-exposed adult rats. Each value represents mean ± SEM of 6 animals. Statistical significance at P < 0.05. a: Control versus others; b: Quercetin versus PCB, PCB+Quer.
Figure 2
Figure 2
Effect of quercetin on Peripheral blood smear of PCBs-exposed adult rats (100X). Peripheral smear study of PCBs-exposed and other group (Leishman's Stain, Nikon Eclipse 80i, 100x oil immersion). Microphotograph of the blood smear of control (a), quercetin (b), PCB (c) and PCB+Quercetin (d) rats. (a), (b), and (d) show the Normochromo normocytic RBCs; leucocytes and platelets are adequate. PCB (c). Total number of RBS are less in number and it shows hypochromo microcytic RBCs, leucocytes are normal, platelets are less in numbers. P: platelets, H: hypochromic microcytic cells, N: neutrophils.
Figure 3
Figure 3
(a) Effect of quercetin on serum liver enzyme activities of PCBs-exposed adult rats. Each bar represents mean ± SEM of 6 animals. Statistical significance at P < 0.05. a: control versus others; b: Quercetin versus PCB, PCB+Quer; c: PCB versus PCB+Quer. AST: aspartate transaminase, ALT: alanine transaminase, ALP: alkaline phosphatase, GGT: gamma glutamyl transaminase. (b) Effect of quercetin on serum amylase, lipase, and CPK activities in PCBs-exposed adult rats. Each bar represents mean ± SEM of 6 animals. Statistical significance at P < 0.05. a: control versus others; b: quercetin versus PCB, PCB+Quer; c: PCB versus PCB+Quer CPK: creatinine phosphokinase.
Figure 4
Figure 4
Liver histology in control, quercetin-, PCB, and PCB+Quercetin-treated adult male rats (Haematoxylin and Eosin staining, ((a), (b), (c), (d) 40x) and ((e), (f), (g), (h) 100x). Photomicrograph of the liver cells ((e)-(f)) Liver sections of control (e) and quer-treated (f) rats showing normal histological appearance of the liver, including portal canal (PC), hepatocytes (H), centrally located nuclei (N), and Kupffer cell (K). (g) Section of rat liver treated with PCB revealed considerable number of damaged hepatocytes (H) that have lost their characteristic appearance, with appearance of Pyknotic Nuclei (PN), apoptotic hepatocytes (bolded arrow) and dilation in blood sinusoids (DBS). (h) Liver section of the rat treated with PCB supplemented with quer demonstrated restoration of normal arrangement of hepatocytes, although few damaged hapatoctes were also observed. (Haemotoxylin and Eosin staining, 40x and 100x).
Figure 5
Figure 5
Effect of quercetin on serum lipid profiles of PCBs-exposed adult rats. Each bar represents mean ± SEM of 6 animals. Statistical significance at P < 0.05. a: control versus others; b: quercetin versus PCB, PCB+Quer; c: PCB versus PCB+Quer.
Figure 6
Figure 6
Effect of quercetin on serum protein metabolism of PCBs-exposed adult rats. Each bar represents mean ± SEM of 6 animals. Statistical significance at P < 0.05. a: control versus others; b: quercetin versus pCB, PCB+Quer; c: PCB versus PCB+Quer.
Figure 7
Figure 7
Effect of quercetin on selected serum electrolytes of PCBs-exposed adult rats. Each bar represents mean ± SEM of 6 animals. Statistical significance at P < 0.05. a: Control versus others; b: Quercetin versus PCB, PCB+Quer; c: PCB versus PCB+Quer.
Figure 8
Figure 8
Effect of quercetin on serum non-protein nitrogenous of PCBs-exposed adult rats. Each bar represents mean ± SEM of 6 animals. Statistical significance at P < 0.05. a: Control versus others; b: Quercetin versus PCB, PCB+Quer; c: PCB versus PCB+Quer.
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