Cellular Response of Stem Cells on Nanofibrous Scaffold for Ocular Surface Bioengineering

Abstract

In this study, the human limbus stem cell was used to evaluate the phenotypic profile, viability, proliferation, and attachment ability of nanofibrous poly (3-hydroxybutyrate-co-3-hydroxyvalerate) substrates. Results indicated that the all substrates were highly biocompatible, as firmly anchored to the substrates, and were able to retain a normal corneal stem cell phenotype. Immunocytochemistry results revealed no change in the expression profile of cells grown on nanofibrous substrate when compared with those grown on amniotic membrane. In addition, nanofibrous substrate provides not only a milieu supporting cells expansion, but also serve as a useful alternative carrier for ocular surface engineering.