Validating subcellular thermal changes revealed by fluorescent thermosensors

Shigeki Kiyonaka^{1

2}, Reiko Sakaguchi³, Itaru Hamachi¹, Takashi Morii⁴, Takenao Yoshizaki⁵, Yasuo Mori^{1

2}

Affiliations

¹ Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan.
² Department of Technology and Ecology, Hall of Global Environmental Studies, Kyoto University, Kyoto, Japan.
³ World Premier International Research Initiative-Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto, Japan.
⁴ Institute of Advanced Energy, Kyoto University, Uji, Japan.
⁵ Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan.

Comment

Shigeki Kiyonaka et al. Nat Methods. 2015 Sep.

. 2015 Sep;12(9):801-2.

doi: 10.1038/nmeth.3548.

Shigeki Kiyonaka^{1

2}, Reiko Sakaguchi³, Itaru Hamachi¹, Takashi Morii⁴, Takenao Yoshizaki⁵, Yasuo Mori^{1

2}

¹ Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan.
² Department of Technology and Ecology, Hall of Global Environmental Studies, Kyoto University, Kyoto, Japan.
³ World Premier International Research Initiative-Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto, Japan.
⁴ Institute of Advanced Energy, Kyoto University, Uji, Japan.
⁵ Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan.

No abstract available

Comment in

A critique of methods for temperature imaging in single cells.
Baffou G, Rigneault H, Marguet D, Jullien L. Baffou G, et al. Nat Methods. 2014 Sep;11(9):899-901. doi: 10.1038/nmeth.3073. Nat Methods. 2014. PMID: 25166869