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Comment
. 2015 Sep;12(9):821-2.
doi: 10.1038/nmeth.3516.

Removing roadblocks to deep sequencing of modified RNAs

Jeremy E Wilusz  1
Affiliations
Comment

Removing roadblocks to deep sequencing of modified RNAs

Jeremy E Wilusz. Nat Methods. 2015 Sep.

Abstract

By removing modified nucleotides that block reverse transcriptase, two methods have now made tRNAs amenable to RNA-seq.

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Conflict of interest statement

COMPETING FINANCIAL INTERESTS

The author declares no competing financial interests.

Figures

Figure 1
Figure 1
Removing methylated nucleotides to sequence tRNAs. Whereas reverse transcriptase (RT) cannot extend through a fully modified tRNA (left), treatment with AlkB removes select methylated nucleotides to allow efficient reverse transcription and deep sequencing (center and right). Full-length and truncated cDNAs are shown in purple and green, respectively.
See this image and copyright information in PMC

Comment on

References

    1. Zheng G, et al. Nat Methods. 2015;12:835–837. - PMC - PubMed
    1. Cozen AE, et al. Nat Methods. 2015;12:879–884. - PMC - PubMed
    1. Wilusz JE. Wiley Interdiscip Rev RNA. 2015;6:453–470. - PMC - PubMed
    1. Gingold H, et al. Cell. 2014;158:1281–1292. - PubMed
    1. Pavon-Eternod M, Gomes S, Rosner MR, Pan T. RNA. 2013;19:461–466. - PMC - PubMed

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