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. 2015 Oct;12(10):955-8.
doi: 10.1038/nmeth.3556. Epub 2015 Aug 31.

Inntags: small self-structured epitopes for innocuous protein tagging

Maya V Georgieva  1 , Galal Yahya  1   2 , Laia Codó  3   4 , Raúl Ortiz  1 , Laura Teixidó  5 , José Claros  6 , Ricardo Jara  6 , Mònica Jara  5 , Antoni Iborra  5 , Josep Lluís Gelpí  3   4   7 , Carme Gallego  1 , Modesto Orozco  4   7   8 , Martí Aldea  1
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Inntags: small self-structured epitopes for innocuous protein tagging

Maya V Georgieva et al. Nat Methods. 2015 Oct.

Abstract

Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.

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References

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