MicroRNA signatures from multidrug‑resistant Mycobacterium tuberculosis

Abstract

Tuberculosis (TB) infections, caused by multidrug‑resistant Mycobacterium tuberculosis (MDR MTB), remain a significant public health concern worldwide. The regulatory mechanisms underlying the emergence of MDR MTB strains remain to be fully elucidated, and further investigation is required in order to develop better strategies for TB control. The present study investigated the expression profile of microRNA (miRNA) in MTB strains, and examined the differences between sensitive MTB and MDR MTB using next generation sequencing (NGS) with Illumina Deep Sequencing technology to better understand the mechanisms of resistance in MDR MTB, A total of 5, 785 and 195, and 6, 290 and 595 qualified Illumina reads were obtained from two MDR MTB strains, and 6, 673 and 665, and 7, 210 and 217 qualified Illumina reads were obtained from two sensitive MTB strains. The overall de novo assembly of miRNA sequence data generated 62 and 62, and 95 and 112 miRNAs between the 18 and 30 bp long from sensitive MTB strains and MDR MTB strains, respectively. Comparative miRNA analysis revealed that 142 miRNAs were differentially expressed in the MDR MTB strain, compared with the sensitive MTB strain, of which 48 were upregulated and 94 were downregulated. There were six similarly expressed miRNAs between the MDR and sensitive MTB strains, and 108 miRNAs were expressed only in the MDR MTB strain. The present study acquired miRNA data from sensitive MTB and MDR MTB strains using NGS techniques, and this identification miRNAs may serve as an invaluable resource for revealing the molecular basis of the regulation of expression associated with the mechanism of drug‑resistance in MTB.

Figure 1

Capacity of sequencing. A total of 5, 785 and 195, and 6, 290 and 595 qualified Illumina reads were obtained from two MDR MTB strains. and 6, 673 and 665, and 7, 210 and 217 were obtained from two sensitive MTB strains. MDR, multi-drug resistant; MTB, Mycobacterium tuberculosis.

Figure 2

Quality of sequencing. According to the statistical results, the average quality of >99% of reads was >20 in each sample. MDR, multi-drug resistant; MTB, Mycobacterium tuberculosis.

Figure 3

Read length distribution. The raw data of next generation sequencing are presented. MDR, multi-drug resistant; MTB, Mycobacterium tuberculosis.

Figure 4

sRNA length distribution. The sRNA tags are shown in the graph. MDR, multi-drug resistant; MTB, Mycobacterium tuberculosis; sRNA, small RNA.

Figure 5

Genome map rate. The graph displays the genome map rate of the MTB samples. MDR, multi-drug resistant; MTB, Mycobacterium tuberculosis.