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. 2015 Nov;59(11):7121-3.
doi: 10.1128/AAC.01815-15. Epub 2015 Aug 31.

Analytical and functional determination of polymyxin B protein binding in serum

Henrietta Abodakpi  1 , Jie Gohlke  1 , Kai-Tai Chang  2 , Diana S-L Chow  1 , Vincent H Tam  3
Affiliations

Analytical and functional determination of polymyxin B protein binding in serum

Henrietta Abodakpi et al. Antimicrob Agents Chemother. 2015 Nov.

Abstract

To enhance our understanding of the pharmacological properties of polymyxin B, serum protein binding for polymyxin B1, B2, and B3 and for isoleucine-polymyxin B1 was evaluated. Using equilibrium dialysis and ultrafiltration, comparable protein binding was found in all 4 components of polymyxin B (92% to 99%). Protein binding in human serum was further assessed using a functional assay, the results of which were in general agreement with previous findings (approximately 90%).

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Figures

FIG 1
FIG 1
Time-killing studies of polymyxin B in 0.5-strength CA-MHB and human serum for P. aeruginosa ATCC 27853 (A) and A. baumannii ATCC BAA 747 (B). The data are shown as means ± standard deviations (SD); MHB, Mueller-Hinton broth.
See this image and copyright information in PMC

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