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. 2015 Nov;59(11):7113-6.
doi: 10.1128/AAC.01394-15. Epub 2015 Aug 31.

Novel plasmid-borne multidrug resistance gene cluster including lsa(E) from a linezolid-resistant Enterococcus faecium isolate of swine origin

Hongbin Si  1 , Wan-Jiang Zhang  2 , Shengbo Chu  2 , Xiu-Mei Wang  2 , Lei Dai  3 , Xin Hua  2 , Zhimin Dong  2 , Stefan Schwarz  4 , Siguo Liu  5
Affiliations

Novel plasmid-borne multidrug resistance gene cluster including lsa(E) from a linezolid-resistant Enterococcus faecium isolate of swine origin

Hongbin Si et al. Antimicrob Agents Chemother. 2015 Nov.

Abstract

A novel nonconjugative plasmid of 28,489 bp from a porcine linezolid-resistant Enterococcus faecium isolate was completely sequenced. This plasmid harbored a novel type of multiresistance gene cluster that comprised the resistance genes lnu(B), lsa(E), spw, aadE, aphA3, and two copies of erm(B), which account for resistance to macrolides, lincosamides, streptogramins, pleuromutilins, streptomycin, spectinomycin, and kanamycin/neomycin. Structural comparisons suggested that this plasmid might have developed from other enterococcal plasmids by insertion element (IS)-mediated interplasmid recombination processes.

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Figures

FIG 1
FIG 1
Linear comparison of the lsa(E)-carrying plasmid pY13 (GenBank accession no. KR936172) with enterococcal and staphylococcal plasmids pXD4, pXD5, pM7M2, pV7037, and p3. Arrows, positions and directions of transcription of the genes; shading, regions of >99% nucleotide sequence identity in the different plasmids; black arrowheads, the nine overlapping PCRs (PCR1 to PCR9) designed to confirm the pY13 plasmid and the genetic environment of the lsa(E) gene; Δ, a truncated gene.
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