Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Sep 1;2015(9):pdb.prot086355.
doi: 10.1101/pdb.prot086355.

Tracking Movements of the Microtubule Motors Kinesin and Dynein Using Total Internal Reflection Fluorescence Microscopy

Ahmet Yildiz, Ronald D Vale
Free article

Tracking Movements of the Microtubule Motors Kinesin and Dynein Using Total Internal Reflection Fluorescence Microscopy

Ahmet Yildiz et al. Cold Spring Harb Protoc. .
Free article

Abstract

Total internal reflection fluorescence microscopy (TIRFM) is a wide-field illumination technique that illuminates only the molecules near the glass coverslip. It has become widely used in biological imaging because it has a significantly reduced background and high temporal resolution capability. The principles of TIRFM are illustrated in this protocol, in which the movements of motor proteins are imaged as they move along microtubules within live axonemes.

PubMed Disclaimer

MeSH terms