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. 2015 Sep;8(3):268-74.
doi: 10.3342/ceo.2015.8.3.268. Epub 2015 Aug 13.

A Higher Angiogenin Expression is Associated With a Nonnuclear Maspin Location in Laryngeal Carcinoma

Andrea Lovato  1 , Marco Lionello  1 , Alberto Staffieri  1 , Stella Blandamura  2 , Giulia Tealdo  1 , Luciano Giacomelli  2 , Claudia Staffieri  3 , Gino Marioni  1
Affiliations

A Higher Angiogenin Expression is Associated With a Nonnuclear Maspin Location in Laryngeal Carcinoma

Andrea Lovato et al. Clin Exp Otorhinolaryngol. 2015 Sep.

Abstract

Objectives: In numerous malignancies, angiogenin (ANG) and Maspin are important proangiogenic and antiangiogenic regulators, respectively. The aim of this study was to identify potential relationships between the biological roles of these two proteins in laryngeal squamous cell carcinoma (LSCC).

Methods: Immunohistochemical staining for ANG and Maspin was performed on specimens from 76 consecutive LSCC patients treated with surgery alone, considering the subcellular pattern of Maspin expression. Univariate and multivariate statistical models were used for prognostic purposes.

Results: On univariate analysis, a different level of ANG expression was seen for patients stratified by subcellular Maspin expression pattern: the mean ANG expression was higher in cases with a nonnuclear MASPIN expression than in those with a nuclear pattern (P=0.002). Disease-free survival (DFS; in months) differed significantly when patients were stratified by N stage (P=0.01). Patients whose Maspin expression was nonnuclear (i.e., it was cytoplasmic or there was none) had a significantly higher recurrence rate (P<0.001), and shorter DFS (P=0.01) than those with a nuclear Maspin pattern. The mean ANG expression was significantly higher in cases with loco-regional recurrent disease (P=0.007); and patients with an ANG expression ≥5.0% had a significantly shorter DFS than those with an ANG expression <5.0% (P=0.007). On multivariate analysis, ANG expression ≥5.0% was a significant, independent, negative prognostic factor in terms of DFS (P=0.041).

Conclusion: Our results support the hypothesis that a higher ANG expression is associated with a nonnuclear Maspin expression pattern in patients with LSCC. Further studies are needed to clarify the relationship between the ANG and Maspin pathways, and their potential diagnostic and therapeutic role in LSCC.

Keywords: Angiogenin; Intracellular Space; Laryngeal Neoplasms; Prognosis; SERPIN-B5.

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Conflict of interest statement

CONFLICT OF INTEREST: No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1
Fig. 1. (A, B) A patient whose laryngeal squamous cell carcinoma recurred, with a high angiogenin (ANG) expression (A), and a cytoplasmic pattern of Maspin expression (B), in carcinoma cells; (C, D) a patient who had no evidence of disease during the follow-up, with a low ANG expression (C), and a nuclear pattern of Maspin expression (D). (A-D) Immunohistochemical staining.
Fig. 2
Fig. 2. (A) Disease-free survival estimates by angiogenin (ANG) expression (%) in laryngeal carcinoma cells (ANG<5.0% or ≥5.0%); (B) disease-free survival estimates by pattern of Maspin expression in laryngeal carcinoma cells (nuclear or nonnuclear); time calculated in months.
Fig. 3
Fig. 3. Square root transformed box plot showing the different angiogenin (ANG) expression in patients stratified by subcellular Maspin expression pattern: mean ANG expression was higher in patients with a nonnuclear Maspin expression pattern (nonnuclear) than in those with a nuclear pattern (nuclear).
See this image and copyright information in PMC

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