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Comparative Study
. 2015 Jul 1;8(7):8305-10.
eCollection 2015.

Diagnostic value of dual detection of hepatocyte nuclear factor 1 beta (HNF-1β) and napsin A for diagnosing ovarian clear cell carcinoma

Qing Li  1 , Xin Zeng  2 , Xue Cheng  2 , Jingmin Zhang  2 , Jie Ji  2 , Jinsong Wang  3 , Kemei Xiong  3 , Qiong Qi  3 , Wenbin Huang  3
Affiliations
Comparative Study

Diagnostic value of dual detection of hepatocyte nuclear factor 1 beta (HNF-1β) and napsin A for diagnosing ovarian clear cell carcinoma

Qing Li et al. Int J Clin Exp Pathol. .

Abstract

We evaluated the diagnostic value of hepatocyte nuclear factor 1 beta (HNF-1β) and napsin A for diagnosing ovarian clear cell carcinoma. Immunohistochemical EnVision was used to measure HNF-1β and napsin A expression in 38 cases of ovarian clear cell carcinoma, 30 cases of high-grade serous carcinoma, 22 cases of endometrioid adenocarcinoma, and 16 metastatic Krukenberg tumor cases. Then we found that HNF-1β appeared in all ovarian clear cell carcinoma and was less common in high-grade serous and endometrioid adenocarcinoma (P < 0.05). However, no significant difference in HNF-1β between clear cell carcinoma and metastatic Krukenberg tumor was found (P > 0.05). Napsin A was expressed in 97.4% of ovarian clear cell carcinoma, 6.7% high-grade serous carcinoma, 22.7% endometrioid adenocarcinoma, and 0% metastatic Krukenberg tumors. Napsin A in clear cell carcinoma was greater than that found in high-grade serous carcinoma, endometrioid adenocarcinoma, and metastatic Krukenberg tumor (P < 0.05). Sensitivity and specificity of HNF-1β and napsin A for diagnosing ovarian clear cell carcinoma was 100% and 54.4%, and 97.4% and 89.7%, respectively. Sensitivity and specificity of HNF-1β and napsin A for diagnosing ovarian clear cell carcinoma was 97.4% and 91.2%, respectively. So it is concluded that HNF-1β and napsin A are more sensitive than currently used markers for diagnosing ovarian clear cell carcinoma. Moreover, napsin A is more specific than HNF-1β. Combining HNF-1β and napsin A may distinguish clear cell carcinoma from high-grade serous carcinoma, endometrioid adenocarcinoma and metastatic Krukenberg tumors.

Keywords: Ovarian neoplasm; clear cell carcinoma; endometrioid adenocarcinoma; hepatocyte nuclear factor 1 beta (HNF-1β); napsin A; serous carcinoma.

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Figures

Figure 1
Figure 1
HNF-1β and napsin A protein expression was observed in the nuclear and cytoplasm of ovarian clear cell carcinoma by EnVision immunohistochemistry. In (A) ovarian clear cell carcinoma, (B) HNF-1β expression was observed in the nuclear, (C) napsin A expression was observed in the cytoplasm (Magnification, × 200).
Figure 2
Figure 2
HNF-1β protein expression was observed in the nuclear of others ovarian neoplasm. In (A) high-grade ovarian serous carcinoma, (B) endometrioid adenocarcinoma, (C) krukenberg tumor (Magnification, × 200).
Figure 3
Figure 3
Napsin A protein expression was observed in the cytoplasm of others ovarian neoplasm. In (A) high-grade ovarian serous carcinoma, (B) endometrioid adenocarcinoma, (C) krukenberg tumor (Magnification, × 200).
See this image and copyright information in PMC

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