Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Oct 18;1(4):463-80.
doi: 10.3390/vaccines1040463.

Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs

Stefano Petrini  1 , Giorgio Ramadori  2 , Riccardo Villa  3 , Paolo Borghetti  4 , Elena de Angelis  5 , Anna Maria Cantoni  6 , Attilio Corradi  6 , Augusto Amici  7 , Maura Ferrari  8
Affiliations

Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs

Stefano Petrini et al. Vaccines (Basel). .

Abstract

In veterinary medicine, there have been different experiences with the plasmid DNA vaccination. In this area and with the hypothesis to demonstrate the effectiveness of different plasmids encoding porcine respiratory and reproductive syndrome (PRRS), five DNA vaccines against PRRS were evaluated for their innocuity and efficacy in pigs. Eighteen animals were divided into five groups which were injected with five (A, B, C, D, E) different DNA vaccines. Albeit, none of the proposed vaccines were able to protect the animals against PRRS virus. Only vaccines A and B were able to reduce the clinical signs of the infection. ELISA IgM were detected 30 days after the first vaccination in the pigs injected by Vaccine A or B. ELISA IgG were detected 90 days after the first vaccination in the pigs injected by Vaccine B or C. Neutralizing antibody were detected Post Challenge Days 61 (PCD) in all groups. In the pigs inoculated with Vaccine C, IFN-g were detected 90 days after first vaccination, and after challenge exposure they increased. In the other groups, the IFN-g were detected after challenge infection. Pigs injected with each of the vaccines A, B, C, D and E showed a significantly higher level of CD4(-)CD8⁺ lymphocytes (p < 0.001) after infection in comparison with their controls.

Keywords: CpG; DNA vaccines; NeuL; ORF4; ORF5; PRRS; UbilacI; pigs.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Schematic genome of porcine reproductive and respiratory syndrome virus (PRRSV) composed of 10 open reading frames (ORFs) encoding the different functional and structural proteins. In particular, ORF4 and ORF5 are used in the plasmid encoding GP4 or GP5 proteins.
Figure 2
Figure 2
Plasmid pVAX1-48CpG-NeuL-ORF4.
Figure 3
Figure 3
Plasmid pVAX1-48CpG-NeuL-ORF5.
Figure 4
Figure 4
Plasmid pVAX1-48CpG-UbilacI-ORF4.
Figure 5
Figure 5
Plasmid pVAX1-48CpG-UbilacI-ORF5.
Figure 6
Figure 6
Plasmid pVAX1-48CpG.
Figure 7
Figure 7
FITC immunofluorescence of mouse embryonic fibroblast cells (NIH-3T3) transfected with pVAX1-48CpG-NeuL-ORF4-Myc. (A) or pVAX1-48CpG-NeuL-ORF5-Myc; (B) after 48 h from transfection.
Figure 8
Figure 8
IFN-γ response (pg/mL) in pigs immunised with experimental PRRS DNA vaccines and challenge infected with virulent PRRSV.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Cavanagh D. Nidovirales: A new order comprising Coronaviridae and Arteriviridae. Arch. Virol. 1997;142:629–633. - PubMed
    1. Costers S., Lefebvre D.J., van Doorsselaere J., Vanhee M., Delputte P.L., Nauwynck H.J. GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro. Arch. Virol. 2010;155:371–378. doi: 10.1007/s00705-009-0582-7. - DOI - PubMed
    1. Vanhee M., van Breedman W., Costers S., Geldhof M., Noppe Y., Nauwynck H. Characterization of antigenic regions in the porcine reproductive and respiratory syndrome virus by the use of peptide-specific serum antibodies. Vaccine. 2011;29:4794–4804. doi: 10.1016/j.vaccine.2011.04.071. - DOI - PubMed
    1. Li J., Murthaugh M.P. Dissociation of porcine reproductive and respiratory syndrome virus neutralization from antibodies specific to major envelope protein surface epitopes. Virology. 2012;433:367–376. doi: 10.1016/j.virol.2012.08.026. - DOI - PubMed
    1. Ren X., Wang M., Yin J., Ren Y., Li G. Heterologous expression of fused genes encoding the glycoprotein 5 from PRRSV: A way for producing functional protein in prokaryotic microorganism. J. Biotechol. 2010;147:130–135. doi: 10.1016/j.jbiotec.2010.03.013. - DOI - PMC - PubMed