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Meta-Analysis
. 2015 Sep;94(36):e1246.
doi: 10.1097/MD.0000000000001246.

Replication and Meta-Analysis of Common Gene Mutations in TTF1 and TTF2 with Papillary Thyroid Cancer

Yan Gao  1 , Fei Chen, Shuli Niu, Shiyu Lin, Suping Li
Affiliations
Meta-Analysis

Replication and Meta-Analysis of Common Gene Mutations in TTF1 and TTF2 with Papillary Thyroid Cancer

Yan Gao et al. Medicine (Baltimore). 2015 Sep.

Abstract

Papillary thyroid cancer (PTC), one of the most common malignant thyroid tumors, exits widely in the thyroid of adolescents. Thyroid transcription factor 1 (TTF1) and 2 (TTF2) were thyroid-specific transcription factors, and regulated expression of the thyroid-specific genes. Hence, the aim of the present study was to evaluate the correlation between gene variants of TTF1 and TTF2 and the risk of PTC in Chinese population.Two tagging single-nucleotide polymorphisms (tSNPs) on TTF1 and TTF2 were selected and genotyped by matrix-assisted laser desorption/ionization time-of-flight (MALDITOF) mass spectrometry in a hospital-based case-control study of 297 PTC patients and 594 healthy controls. Furthermore, a meta-analysis of the association between TTF1 and TTF2 and PTC risk was also performed.We found that the rs944289 on the TTF1 was significantly associated with increased PTC risk (TT vs CC, OR = 1.53, 95% CI = 1.05-2.24; CT + TT vs TT, OR = 1.34, 95% CI = 1.00-1.79; T vs C, OR = 1.27, 95% CI = 1.04-1.55). Similarly, the rs965513 on the TTF2 can also elevate the risk of PTC significantly (GA vs GG, OR = 1.67, 95% CI = 1.07-2.59; AA+GA vs AA, OR = 1.37, 95% CI = 1.09-1.82; A vs G, OR = 1.29, 95% CI = 1.05-1.59). Furthermore, results of stratified analysis revealed that the risk effects of rs944289 and rs965513 were more overpowering in the subgroups of patients with MNG, as well as subjects without metastasis. Results of meta-analysis from the previous study and our new data indicated that variants of rs944289 and rs965513 might be the genetic susceptible factors both in Asians and Caucasians.We get the conclusion that mutations of TTF1 and TTF2 are significantly associated with an increasing risk of PTC in Chinese. However, more detailed investigations and further large-scale studies on genetic functions to provide more conclusive and accurate evidence are required in the future.

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Conflict of interest statement

The authors had full access to all data in the study and had final responsibility for the decision to submit for publication. The authors have no conflicts of interest to disclose.

Figures

FIGURE 1
FIGURE 1
Forest plot for the meta-analysis of the association between rs944289 mutation and PTC.
FIGURE 2
FIGURE 2
Forest plot for the meta-analysis of the association between rs965513 mutation and PTC.
FIGURE 3
FIGURE 3
Funnel plot analysis to detect publication bias for rs944289 (a) and (b) rs965513.
FIGURE 4
FIGURE 4
Mechanism between TTF1 and TTF2 gene variations and thyroid cancer.
See this image and copyright information in PMC

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