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Published Erratum
. 2015 Sep;27(9):2664-5.
doi: 10.1105/tpc.15.00761. Epub 2015 Sep 11.

Correction

No authors listed
Published Erratum

Correction

No authors listed. Plant Cell. 2015 Sep.
No abstract available

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Figures

Figure 1.
Figure 1.
Original: PUB13 Interacts Specifically with the Active Form of RabA4B. (A) Y2H interaction of PUB13Δ552-660 with active GTP-bound RabA4B (T), but not inactive GDP-bound RabA4B (D), was detected on high-stringency medium (−HisTrpLeu + 3-aminotriazole [−HTL+3-AT]). No interaction was observed with RabF2A, RabG3C, and ROP1. The presence of prey and/or bait vectors was monitored by growth in the absence of leucine and tryptophan (−LT) or tryptophan (−T), respectively. (B) UND, U-box, and ARM domains are indicated. Deletion fragments of PUB13 were constructed to determine the binding site of RabA4B. (C) Y2H interaction was seen between active RabA4B and PUB13 fragments on selective medium (−HisTrpLeu+3-AT). The interaction between RabA4B and PUB13 requires the presence of UND and U-box domains. No interaction was observed between RabA4B and the ARM domain. Surprisingly, full-length PUB13 did not interact with RabA4B in the Y2H assay. (Inadvertently duplicated spots are circled in red).
Figure 1.
Figure 1.
Corrected: PUB13 Interacts Specifically with the Active Form of RabA4B. (A) Y2H interaction of PUB13Δ552-660 with active GTP-bound RabA4B (T), but not inactive GDP-bound RabA4B (D), was detected on high-stringency medium (−HisLeuTrp + 3-aminotriazole [−HLT+3-AT]). No interaction was observed with either form of RabF2A, RabG3C, and ROP1. The presence of prey and/or bait vectors was monitored by growth in the absence of leucine and tryptophan (−LT) or tryptophan (−T), respectively. Bait “T” and prey “p53” represent positive controls for interaction. (B) UND, U-box, and ARM domains are indicated. Deletion fragments of PUB13 were constructed to determine the binding site of RabA4B. (C) Y2H interaction was seen between active RabA4B (T) and PUB13 fragments on selective medium (−HLT+3-AT). The interaction between RabA4B and PUB13 requires the presence of UND and U-box domains. No interaction was observed between RabA4B and the ARM domain. Surprisingly, full-length PUB13 did not interact with RabA4B in the Y2H assay. Yeast spots grown in each selection condition were all from the same plate but rearranged in the figure for a logical order. Positive control as in (A). (Images that were replaced relative to the original figure are underlined in red).
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