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. 1989 Dec;135(12):3303-9.
doi: 10.1099/00221287-135-12-3303.

Cloning of a thermostable alpha-amylase gene from Thermomonospora curvata and its expression in Streptomyces lividans

M Petrícek  1 , K Stajner, P Tichý
Affiliations

Cloning of a thermostable alpha-amylase gene from Thermomonospora curvata and its expression in Streptomyces lividans

M Petrícek et al. J Gen Microbiol. 1989 Dec.

Abstract

The gene from Thermomonospora curvata CCM 3312 coding for thermostable alpha-amylase (tam) has been cloned in Streptomyces lividans TK 24 and localized to a 2.6 kb HindIII-BamHI fragment of DNA. The data presented here show that the tam gene is expressed at a high level in S. lividans and that the protein is efficiently excreted.

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