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. 2016 Jan;26(1):139-42.
doi: 10.1038/cr.2015.112. Epub 2015 Sep 15.

Generation of macaques with sperm derived from juvenile monkey testicular xenografts

Zhen Liu  1 , Yan-Hong Nie  1 , Chen-Chen Zhang  1 , Yi-Jun Cai  1 , Yan Wang  1 , Hui-Ping Lu  1 , Yu-Zhuo Li  1 , Cheng Cheng  1 , Zi-Long Qiu  1 , Qiang Sun  1
Affiliations

Generation of macaques with sperm derived from juvenile monkey testicular xenografts

Zhen Liu et al. Cell Res. 2016 Jan.
No abstract available

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Figures

Figure 1
Figure 1
Generation of monkey offspring using sperm derived from juvenile monkey testicular xenografts. (A) Testicular tissue sections from a 14-month-old WT monkey before xenografting (top) and 10 months after the xenografting (bottom). Scale, 1 mm. (B) Xenografts of the WT testis 4 months after subcutaneous implantation in nude mice. Arrow points to one xenograft. (C) Sperm collected from the TG monkey testicular tissue xenograft 11 months after the implantation. Scale bar, 20 μm. (D–I) Sample sections of xenografts from the WT and TG monkey testicular tissues at different times (marked as T). Small seminiferous tubules were seen prior to the grafting (D, G). Growth of the tubules was observed in the xenografts (E, H), and mature sperms (marked by arrows) were observed after 10 months (F, I). Scale bar, 100 μm. (J) Monkey XG1 obtained by using fresh sperms from the xenograft of the WT monkey donor. (K) Monkey XG6 obtained by using freeze-thawed sperms from xenografts of the TG monkey donor. (L) PCR analysis of skin tissue samples of the F1 infant monkeys showing the presence of GFP in 5 TG monkeys but not in 2 WT monkeys. (M) Detection of the GFP protein in the brain tissue of the XG2 monkey which died 3 days after birth using western blotting. (N) Summary of the efficiency of generating XG monkeys. MC, miscarried; ND, no data; *, activated with ionomycin and CHX.
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