Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Sep 9;16(9):21695-710.
doi: 10.3390/ijms160921695.

CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of "Wuzishatangju" (Citrus reticulata Blanco)

Peng Li  1 , Hongxia Miao  2   3 , Yuewen Ma  4 , Lu Wang  5 , Guibing Hu  6 , Zixing Ye  7 , Jietang Zhao  8 , Yonghua Qin  9
Affiliations

CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of "Wuzishatangju" (Citrus reticulata Blanco)

Peng Li et al. Int J Mol Sci. .

Abstract

Plant S-phase kinase-associated protein 1 (SKP1) genes play crucial roles in plant development and differentiation. However, the role of SKP1 in citrus is unclear. Herein, we described a novel SKP1-like gene, designated as CrWSKP1, from "Wuzishatangju" (Citrus reticulata Blanco). The cDNA sequence of CrWSKP1 is 779 base pairs (bp) and contains an open reading frame (ORF) of 477 bp. The genomic sequence of the CrWSKP1 gene is 1296 bp with two exons and one intron. CrWSKP1 has high identity with SKP1-like genes from other plant species within two conserved regions. Approximately 85% of pollen tubes of self-pollinated CrWSKP1 transgenic tobaccos became twisted at four days after self-pollination. Pollen tube numbers of self-pollinated CrWSKP1 transformants entering into ovules were significantly fewer than that of the control. Seed number of self-pollinated CrWSKP1 transformants was significantly reduced. These results suggested that the CrWSKP1 is involved in the self-incompatibility (SI) reaction of "Wuzishatangju".

Keywords: Citrus reticulata Blanco; SKP1; genetic transformation; self-incompatibility.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Analyses of CrWSKP1 and CrYSKP1 genes. (A) Alignments of cDNA sequences of CrWSKP1 and CrYSKP1 genes between “Wuzishatangju” (WZSTJ) and “Shatangju” (STJ); (B) Exon-introns structure of CrWSKP1 and CrYSKP1 genes. CrWSKP1, DNA sequence of the Skp1-like gene from “Wuzishatangju”; CrYSKP1, DNA sequence of the Skp1-like gene from “Shatangju”. Solid boxes indicate exons, and bold lines represent introns; (C) Alignments of partial DNA sequences of CrWSKP1 and CrYSKP1 genes between “Wuzishatangju” (WZSTJ) and “Shatangju” (STJ); (D) Alignments of the putative amino acid sequences of CrWSKP1 and CrYSKP1 genes from different plant species. CrWSKP1 (“Wuzishatangju”), CrYSKP1 (“Shatangju”), CzSSKP1-like (Citrus maxima, ACP20181), CaSKP1-like (Cicer arietinum, XP004512164), GmSKP1-like (Glycine max, XP003517160), VvSKP1-like (Vitis vinifera, XP002279232), AtSK20 (Arabidopsis thaliana, A8MQG7), AtSK21 (Arabidopsis thaliana, Q8LF97). Higher conserved regions were marked in box with Skp1_POZ and Skp1.
Figure 2
Figure 2
Phylogenetic relationship of CrWSKP1 and CrYSKP1 with SKP1-like genes from other plants. The numbers of each interior branch are the percentage bootstrap values (re-sampling). CrWSKP1 (“Wuzishatangju”), CrYSKP1 (“Shatangju”), CzSKP1-like (Citrus maxima, ACP20181), CaSKP1-like (Cicer arietinum, XP004512164), GmSKP1-like (Glycine max, XP003517160), VvSKP1-like (Vitis vinifera, XP002279232), CsSKP1-like (Cucumis sativus, XP004144851), CmSKP1-like (Cucumis melo, XP008447650), StSKP1-like (Solanum tuberosum, XP006362720), MtSKP1-like (Medicago truncatula, XP003612227), RcSKP1 (Ricinus communis, XP002510577), MdSKP1-like (Malus domestica, XP008357122), FvSKP1-like (Fragaria vesca subsp. Vescal (XP004287783), AtSK20 (Arabidopsis thaliana, A8MQG7), AtSK21 (Arabidopsis thaliana, Q8LF97).
Figure 3
Figure 3
Southern analysis of Skp1-like genes in (A) “Wuzishatangju” and (B) “Shatangju” mandarins.
Figure 4
Figure 4
Pollination and fertilization of CrWSKP1 transgenic tobacco at different stages S. (A1–A6) WT×WT; (B1–B6) CrWSKP1×CrWSKP1; (C1–C6), CrWSKP1×WT; 1, 2, 3, 4, 5, 6: 1, 2, 3, 4, 5, 6 represent days after self- and cross-pollination. Scale bar = 100 μm.
Figure 5
Figure 5
Seed number per fruit of CrWSKP1 transgenic tobacco after self- or cross-pollination. The vertical bars represent standard deviation (±SD) of three biological replicates. a,b,c represent significant difference using the LSD test at p ≤ 0.05.
See this image and copyright information in PMC

References

    1. Ye W.J., Qin Y.H., Ye Z.X., Teixeira da Silva J.A., Zhang L.X., Wu X.Y., Lin S.Q., Hu G.B. Seedless mechanism of a new mandarin cultivar “Wuzishatangju” (Citrus reticulata Blanco) Plant Sci. 2009;177:19–27. doi: 10.1016/j.plantsci.2009.03.005. - DOI
    1. Distefano G., Caruso M., Malfa S.L., Gentile A., Tribulato E. Histological and molecular analysis of pollen-pistil interaction in clementine. Plant Cell Rep. 2009;28:1439–1451. doi: 10.1007/s00299-009-0744-9. - DOI - PubMed
    1. Ngo B.X., Wakana A., Kim J.H., Mori T., Sakai K. Estimation of self-incompatibility S genotypes of Citrus cultivars and plants based on controlled pollination with restricted number of pollen grains. J. Fac. Agric. Kyushu Univ. 2010;55:67–72.
    1. Caruso M., Merelo P., Distefano G., La Malfa S., Lo Piero A.R., Tadeo F.R., Talon M., Gentile A. Comparative transcriptome analysis of stylar canal cells identifies novel candidate genes implicated in the self-incompatibility response of Citrus clementina. BMC Plant Biol. 2012;1 doi: 10.1186/1471-2229-12-20. - DOI - PMC - PubMed
    1. Mesejo C., Yuste R., Martínez-Fuentes A., Reig C., Iglesias D.J., Primo-Millo E., Agustí M. Self-pollination and parthenocarpic ability in developing ovaries of self-incompatible Clementine mandarins (Citrus clementina) Physiol. Plant. 2013;148:87–96. doi: 10.1111/j.1399-3054.2012.01697.x. - DOI - PubMed

Publication types

Substances

LinkOut - more resources