Isoenzymes of N-acetyl-beta-D-glucosaminidase in urine of patients with acute renal failure after intoxication

Abstract

Activity of urinary N-acetyl-beta-D-glucosaminidase (NAG) was determined and basic clinical studies were carried out in oliguric, polyuric phases and restoration to health in patients with acute renal failure due to intoxication. At the same time, enzyme separation into isoenzymes A and B was achieved by column ion-exchange chromatography and by electrophoresis. This was done in urines from 9 patients and a control group of 10 persons who had no contact with toxic substances. The contribution of particular isoenzymes in total activity was determined by the "batch-wise" method with DEAE-52 cellulose. Significant differences were discovered in thermostability of NAG isoenzymes which may be utilized in nephrological diagnosis.