Early detection of extended-spectrum β-lactamase from blood culture positive for an Enterobacteriaceae using βLACTA test

Guy Prod'hom¹, Christian Durussel¹, Dominique Blanc², Antony Croxatto¹, Gilbert Greub¹

Affiliations

¹ Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
² Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland ; Service of Hospital Preventive Medicine, University of Lausanne and University Hospital Center, Lausanne, Switzerland.

PMID: 26380714
PMCID: PMC4556769
DOI: 10.1016/j.nmni.2015.05.007

Early detection of extended-spectrum β-lactamase from blood culture positive for an Enterobacteriaceae using βLACTA test

Guy Prod'hom et al. New Microbes New Infect. 2015.

. 2015 Jun 9:8:1-3.

doi: 10.1016/j.nmni.2015.05.007. eCollection 2015 Nov.

Authors

Guy Prod'hom¹, Christian Durussel¹, Dominique Blanc², Antony Croxatto¹, Gilbert Greub¹

Affiliations

¹ Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
² Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland ; Service of Hospital Preventive Medicine, University of Lausanne and University Hospital Center, Lausanne, Switzerland.

PMID: 26380714
PMCID: PMC4556769
DOI: 10.1016/j.nmni.2015.05.007

Abstract

Bacterial pellets from Enterobacteriaceae positive blood cultures prepared using ammonium chloride were tested for rapid detection of β-lactamase using the commercial βLACTA test and read after 30 minutes. During 7 months, 137 bacterial pellets were tested prospectively. βLACTA test exhibited a sensitivity of 75% and a specificity of 100% for the detection of third-generation cephalosporin resistance. False negative tests were mainly observed with hyperproduced chromosomal or plasmid-borne AmpC.

Keywords: Ammonium chloride; blood culture; cephalosporins; chromogenic cephalosporin; rapid testing; β-lactamase.

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Early detection of extended-spectrum β-lactamase from blood culture positive for an Enterobacteriaceae using βLACTA test

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Early detection of extended-spectrum β-lactamase from blood culture positive for an Enterobacteriaceae using βLACTA test

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Abstract

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