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. 2015 Sep 17:15:86.
doi: 10.1186/s12935-015-0237-6. eCollection 2015.

Down-regulation of miR-133a and miR-539 are associated with unfavorable prognosis in patients suffering from osteosarcoma

Alireza Mirghasemi  1 , Afshin Taheriazam  2 , Seyyed Hasan Karbasy  3 , Ali Torkaman  4 , Mohammadreza Shakeri  5 , Emad Yahaghi  6 , Aram Mokarizadeh  7
Affiliations

Down-regulation of miR-133a and miR-539 are associated with unfavorable prognosis in patients suffering from osteosarcoma

Alireza Mirghasemi et al. Cancer Cell Int. .

Retraction in

Abstract

Background: MicroRNAs (miRNAs) play key roles in cancer development and progression. The purpose of the present study was to determine the expression levels of miR-133a and miR-539 in osteosarcoma patients and to further investigate the clinicopathological, and prognostic value of these miRNAs.

Methods: The expression levels of miR-133a and miR-539 were determined by qRT-PCR. Associations between miRNAs expressions and various clinicopathological characteristics were analyzed. Survival rate was determined with Kaplan-Meier and statistically analyzed with the log-rank method between groups. Survival data were evaluated through multivariate Cox regression analysis.

Results: Our findings revealed that the miR-133a expression was significantly decreased in clinical osteosarcoma tissues compared to adjacent normal bone tissues. The expression level of miR-539 was decreased in clinical osteosarcoma tissues as compared to those adjacent normal tissues. Low expressions of miR-133a and miR-539 were significantly association with advanced TNM stage (P = 0.002; P = 0.001), and metastasis or recurrence (P = 0.001; P = 0.01). Furthermore, Kaplan-Meier survival analysis and log-rank test showed that the low expressions of miR-133a and miR-539 were correlated with the reduced overall survival of osteosarcoma patients. Multivariate Cox proportional hazards model showed that decreased expressions of miR-133a and miR-539 (P = 0.007; P = 0.02), TNM stage (P = 0.001; P = 0.002), and metastasis or recurrence (P = 0.005; P = 0.026) were independent prognostic markers of overall survival of patients.

Conclusion: These results suggest that decreased miR-133a and miR-539 expressions may participate in the progression of osteosarcoma. Together, these results showed that miR-133a and miR-539 may have their role in both progression and prognosis of osteosarcoma.

Keywords: Cancer; Marker; MicRNA; Patient; Survival.

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Figures

Fig. 1
Fig. 1
The relative expression level of miR-133a between osteosarcoma bone tissue and noncancerous bone tissues
Fig. 2
Fig. 2
The relative expression level of miR-539 between osteosarcoma bone tissue and noncancerous bone tissues
Fig. 3
Fig. 3
Correlation between miR-133a expression and survival in patients
Fig. 4
Fig. 4
Correlation between miR-539 expression and survival in patients
See this image and copyright information in PMC

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