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. 2015 Dec:119:1-3.
doi: 10.1016/j.mimet.2015.09.010. Epub 2015 Sep 25.

A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

La'Tonzia L Adams  1 , Parichat Salee  2 , Kim Dionne  3 , Karen Carroll  3 , Nicole Parrish  3
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A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

La'Tonzia L Adams et al. J Microbiol Methods. 2015 Dec.

Abstract

Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard.

Keywords: MALDI-ToF MS; Mycobacterium; Protein extraction.

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