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. 2015 Sep 22:15:641.
doi: 10.1186/s12885-015-1650-x.

Secreted frizzled-related protein promotors are hypermethylated in cutaneous squamous carcinoma compared with normal epidermis

Junqin Liang  1 , Xiaojing Kang  2 , Yilinuer Halifu  3 , Xuewen Zeng  4 , Tianbo Jin  5   6 , Mingxia Zhang  7 , Dong Luo  8 , Yuan Ding  9 , Yunmin Zhou  10 , Buwajier Yakeya  11 , Dilinuer Abudu  12 , Xiongming Pu  13
Affiliations

Secreted frizzled-related protein promotors are hypermethylated in cutaneous squamous carcinoma compared with normal epidermis

Junqin Liang et al. BMC Cancer. .

Abstract

Background: The Wnt signaling pathway is abnormally activated in many human cancers. Secreted frizzled-related proteins (SFRPs) function as negative regulators of Wnt signaling and play an important role in carcinogenesis. SFRP promoter hypermethylation has often been identified in human cancers; however, the precise role of SFRPs in cutaneous squamous cell carcinoma (SCC) is unclear.

Methods: The methylation status of the SFRP family was analyzed in an age-and sex-matched case-control study, including 40 cutaneous SCC cases and 40 normal controls, using the MassARRAY EpiTYPER system.

Results: The methylation rate of SFRP1, SFRP2, SFRP4, and SFRP5 promoters was significantly higher in cutaneous SCC tissues than in adjacent tissue and normal skin samples.

Discussion: Our manuscript mainly discussed the average methylation rate of SFRPs (SFRP1, SFRP2, SFRP4, and SFRP5) promoters are significantly high in tumor tissue samples and the average CpG island methylation rate among different pathological levels of cutaneous SCC between these genes are different.

Conclusions: Our findings suggest that promoter hypermethylation of SFRPs is associated with the development of carcinoma, and could be a useful tumor marker for cutaneous SCC and other types of cancers.

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Figures

Fig. 1
Fig. 1
Comparison of average CpG island methylation rate in the SFRP gene family. T, tumor tissues; A, adjacent to carcinoma tissue; N, normal tissue samples
Fig. 2
Fig. 2
Receiver operating characteristic (ROC) curves for six CpG sites in the SFRP1 promoter. The ROC curves plot sensitivity and 1-specificity. Areas under the curve (AUC) and P values were shown in the graph
See this image and copyright information in PMC

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