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. 2015 Oct;21(10):1816-9.
doi: 10.3201/eid2110.150840.

Utility of Oral Swab Sampling for Ebola Virus Detection in Guinea Pig Model

Jessica R Spengler, Ayan K Chakrabarti, JoAnn D Coleman-McCray, Brock E Martin, Stuart T Nichol, Christina F Spiropoulou, Brian H Bird

Utility of Oral Swab Sampling for Ebola Virus Detection in Guinea Pig Model

Jessica R Spengler et al. Emerg Infect Dis. 2015 Oct.

Abstract

To determine the utility of oral swabs for diagnosing infection with Ebola virus, we used a guinea pig model and obtained daily antemortem and postmortem swab samples. According to quantitative reverse transcription PCR analysis, the diagnostic value was poor for antemortem swab samples but excellent for postmortem samples.

Keywords: Ebola; guinea pig; oral swab; qRT-PCR; viral hemorrhagic fever; viruses; wild-type Ebola virus.

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Figures

Figure
Figure
Clinical course of guinea pigs infected with guinea pig–adapted Ebola virus (GP-EBOV), wild-type EBOV Makona, and wild-type EBOV Mayinga, by number of days postinfection. A) Percentage of animals that survived. B) Subcutaneous microchip temperature. Dotted line indicates upper limit of reference temperature range for guinea pigs. C) Weight loss from 0 days postinfection.
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