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. 2015 Oct;21(10):1853-7.
doi: 10.3201/eid2110.150678.

Detection of Mixed Infections with Plasmodium spp. by PCR, India, 2014

Sri Krishna, Praveen K Bharti, Himashu S Chandel, Amreen Ahmad, Rajesh Kumar, Puspendra P Singh, Mrigendra P Singh, Neeru Singh

Detection of Mixed Infections with Plasmodium spp. by PCR, India, 2014

Sri Krishna et al. Emerg Infect Dis. 2015 Oct.

Abstract

In 8 malaria-endemic states in India, mixed Plasmodium spp. infections were detected by PCR in 17.4% (265/1,521) of blood samples that microscopy had shown to contain only P. falciparum. The quality of microscopy must be improved because use of PCR for detection of malaria parasites is limited in rural areas.

Keywords: India; PCR; Plasmodium falciparum; Plasmodium spp.; malaria; mixed infections; parasites.

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Figures

Figure 1
Figure 1
Fifteen community health centers in 8 states in India to which malaria is endemic. 1, Udaipur; 2, Dahod; 3, Valsad; 4, Jhabua; 5, Annupur; 6, Gondia; 7, Gadchiroli; 8, Jagdalpur; 9, Baikunthpur; 10, Koraput; 11, Rayagada; 12, Jaldega; 13, Bano; 14, Manu Bazar; 15, Shantir Bazar.
Figure 2
Figure 2
Identification of Plasmodium spp. by nested PCR at 15 community health centers in 8 states in India to which malaria is endemic. A) Plasmodium falciparum (205-bp fragment). Lane 1, molecular mass marker; lane 2, negative (–) control; lane 3, positive (+) control; lanes 7−27, positive samples; lanes 5 and 6, negative samples. B) P. malariae (144-bp fragment). Lane 25, + control; lane 26, – control; lane 27, molecular mass marker; lane 12, positive sample; lanes 1–11, 13–24, negative samples. C) P. ovale (800-bp fragment). Lane 1, molecular mass marker; lane 2, – control; lane 3, + control; lane 17, positive sample; lanes 4–16, 18–27, negative samples. D) P. vivax (120-bp fragment). Lane 1, molecular mass marker; lane 2, + control; lane 3, – control; lanes 5, 11, 16, and 25, positive samples; lanes 4, 6–10, 12–15, 17–24, 26, and 27, negative samples.
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References

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