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. 2015 Sep;24(3):206-10.
doi: 10.5607/en.2015.24.3.206. Epub 2015 Sep 22.

Membrane-Introduction Mass Spectrometry Analysis of Desflurane, Propofol and Fentanyl in Plasma and Cerebrospinal Fluid for Estimation BBB Properties

Vyacheslav Yu Cherebillo  1 , Andrei Yu Elizarov  2 , Andrei V Polegaev  1
Affiliations

Membrane-Introduction Mass Spectrometry Analysis of Desflurane, Propofol and Fentanyl in Plasma and Cerebrospinal Fluid for Estimation BBB Properties

Vyacheslav Yu Cherebillo et al. Exp Neurobiol. 2015 Sep.

Abstract

A possibility to use the Membrane-Introduction Mass Spectrometry (MIMS) with membrane separator interface has evolved into a powerful method for measurement of anaesthetic agents absolute concentration in blood plasma and cerebrospinal fluid for the study of blood-brain barrier (BBB) properties. Recent advanced a new membrane material was used for drug concentration measurement in biologic fluids. A hydrophobic membrane was used in the interface to separate anaesthetic agents from biological fluids: inhalational anaesthetic desflurane,hypnotic propofol, analgesic fentanyl. The selective detection of volatile anesthetic agents in blood does not require long-term sample processing before injecting the sample into mass-spectrometer interface, in contrast to chromatographic methods. Mass-spectrometric interface for the measurement of anaesthetic agent concentration in biological fluids (blood plasma and cerebrospinal fluid) is described. Sampling of biological fluids was performed during balanced inhalational (desflurane, fentanyl) anaesthesia and total intravenous (propofol, fentanyl) anaesthesia.

Keywords: anesthesia; desflurane; fentanyl; mass-spectrometer; membrane.

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Figures

Fig. 1
Fig. 1. Mass-spectrum of desflurane and desflurane in the plasma that was taken during balanced inhalational anaesthesia, obtained using membrane separator interface. Fig. 1a Ratio of drug concentrations in a peripheral vein and in the brain where N (arb. un.) is the ratio concentrations of the anaesthetic agents in PV and in chiasmo-sellar area (cerebrospinal fluid), respectively (see details in the text).
Fig. 2
Fig. 2. Mass-spectrum of fentanyl in the plasma that was taken during balanced inhalational anaesthesia, obtained using MIMS.
Fig. 3
Fig. 3. Mass spectra of propofol (Fresenius Kabi) and blood plasma sample taken during total intravenous propofol-fentanyl anesthesia, measured using MIMS.
Fig. 4
Fig. 4. Mass-spectra of propofol in the plasma that was taken during total intravenous propofol-fentanyl anaesthesia, obtained using MIMS.
Fig. 5
Fig. 5. "UP"-The MIMS Mass spectrum of sevoflurane-fentanyl in the urine that was taken during sevoflurane-fentanyl anaesthesia. "Middle"-the MIMS mass spectrum of evaporation through a membrane fabric (tissue) pituitary adenoma. "Bottom"-the MIMS mass spectrum of desflurane in the blood that was taken during desflurane-fentanyl anaesthesia.
See this image and copyright information in PMC

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