Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Sep;24(3):206-10.
doi: 10.5607/en.2015.24.3.206. Epub 2015 Sep 22.

Membrane-Introduction Mass Spectrometry Analysis of Desflurane, Propofol and Fentanyl in Plasma and Cerebrospinal Fluid for Estimation BBB Properties

Vyacheslav Yu Cherebillo  1 , Andrei Yu Elizarov  2 , Andrei V Polegaev  1
Affiliations

Membrane-Introduction Mass Spectrometry Analysis of Desflurane, Propofol and Fentanyl in Plasma and Cerebrospinal Fluid for Estimation BBB Properties

Vyacheslav Yu Cherebillo et al. Exp Neurobiol. 2015 Sep.

Abstract

A possibility to use the Membrane-Introduction Mass Spectrometry (MIMS) with membrane separator interface has evolved into a powerful method for measurement of anaesthetic agents absolute concentration in blood plasma and cerebrospinal fluid for the study of blood-brain barrier (BBB) properties. Recent advanced a new membrane material was used for drug concentration measurement in biologic fluids. A hydrophobic membrane was used in the interface to separate anaesthetic agents from biological fluids: inhalational anaesthetic desflurane,hypnotic propofol, analgesic fentanyl. The selective detection of volatile anesthetic agents in blood does not require long-term sample processing before injecting the sample into mass-spectrometer interface, in contrast to chromatographic methods. Mass-spectrometric interface for the measurement of anaesthetic agent concentration in biological fluids (blood plasma and cerebrospinal fluid) is described. Sampling of biological fluids was performed during balanced inhalational (desflurane, fentanyl) anaesthesia and total intravenous (propofol, fentanyl) anaesthesia.

Keywords: anesthesia; desflurane; fentanyl; mass-spectrometer; membrane.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1. Mass-spectrum of desflurane and desflurane in the plasma that was taken during balanced inhalational anaesthesia, obtained using membrane separator interface. Fig. 1a Ratio of drug concentrations in a peripheral vein and in the brain where N (arb. un.) is the ratio concentrations of the anaesthetic agents in PV and in chiasmo-sellar area (cerebrospinal fluid), respectively (see details in the text).
Fig. 2
Fig. 2. Mass-spectrum of fentanyl in the plasma that was taken during balanced inhalational anaesthesia, obtained using MIMS.
Fig. 3
Fig. 3. Mass spectra of propofol (Fresenius Kabi) and blood plasma sample taken during total intravenous propofol-fentanyl anesthesia, measured using MIMS.
Fig. 4
Fig. 4. Mass-spectra of propofol in the plasma that was taken during total intravenous propofol-fentanyl anaesthesia, obtained using MIMS.
Fig. 5
Fig. 5. "UP"-The MIMS Mass spectrum of sevoflurane-fentanyl in the urine that was taken during sevoflurane-fentanyl anaesthesia. "Middle"-the MIMS mass spectrum of evaporation through a membrane fabric (tissue) pituitary adenoma. "Bottom"-the MIMS mass spectrum of desflurane in the blood that was taken during desflurane-fentanyl anaesthesia.
See this image and copyright information in PMC

References

    1. Hunger K, Schmeling N, Jeazet HB, Janiak C, Staudt C, Kleinermanns K. Investigation of cross-linked and additive containing polymer materials for membranes with improved performance in pervaporation and gas separation. Membranes (Basel) 2012;2:727–763. - PMC - PubMed
    1. Ojala M, Mattila I, Särme T, Ketola RA, Kotiaho T. A new purge-and-membrane mass spectrometric (PAM-MS) instrument for analysis of volatile organic compounds in soil samples. Analyst. 1999;124:1421–1424.
    1. Johnson RC, Cooks RG, Allen TM, Cisper ME, Hemberger PH. Membrane introduction mass spectrometry: trends and applications. Mass Spectrom Rev. 2000;19:1–37. - PubMed
    1. Saracco G, Neomagus HW, Versteeg GF, van Swaaij WP. High-temperature membrane reactors: potential and problems. Chem Eng Sci. 1999;54:1997–2017.
    1. Bolto B, Hoang M, Xie ZL. A review of membrane selection for the dehydration of aqueous ethanol by pervaporation. Chem Eng Process. 2011;50:227–235.

LinkOut - more resources