Genome sequence of Anoxybacillus ayderensis AB04(T) isolated from the Ayder hot spring in Turkey

Abstract

Species of Anoxybacillus are thermophiles and, therefore, their enzymes are suitable for many biotechnological applications. Anoxybacillus ayderensis AB04(T) (= NCIMB 13972(T) = NCCB 100050(T)) was isolated from the Ayder hot spring in Rize, Turkey, and is one of the earliest described Anoxybacillus type strains. The present work reports the cellular features of A. ayderensis AB04(T), together with a high-quality draft genome sequence and its annotation. The genome is 2,832,347 bp long (74 contigs) and contains 2,895 protein-coding sequences and 103 RNA genes including 14 rRNAs, 88 tRNAs, and 1 tmRNA. Based on the genome annotation of strain AB04(T), we identified genes encoding various glycoside hydrolases that are important for carbohydrate-related industries, which we compared with those of other, sequenced Anoxybacillus spp. Insights into under-explored industrially applicable enzymes and the possible applications of strain AB04(T) were also described.

Keywords: Anoxybacillus; Bacillaceae; Bacillus; Geobacillus; Glycoside hydrolase; Thermophile.

Fig. 1

FESEM micrograph of A. ayderensis AB04^T. The micrograph was captured using FESEM (JEOL JSM-6701 F, Tokyo, Japan) operating at 5.0 kV at a magnification of 15,000 ×

Fig. 2

Phylogenetic tree based on 16S rRNA gene sequences showing the relationship between A. ayderensis AB04^T and representative Anoxybacillus spp. The 16S rRNA accession number for each strain is shown in brackets. The 16S rRNA sequences were aligned using ClustalW and the tree was constructed using the ML method with 1000 bootstrap replicates embedded in the MEGA6.0 package [49]. The scale bar represents 0.01 nucleotide substitutions per position. Brevibacillus brevis NCIMB 9372^T [77] was used as an out-group. Type strains are indicated with a superscript T. Published genomes are indicated in blue

Fig. 3

A graphical circular map of the A. ayderensis AB04^T genome. From outside to the center: genes on the forward strand (colored by COG categories), genes on forward strand (red), genes on reverse strand (blue) and genes on the reverse strand (colored by COG categories)