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. 2015 Jul-Dec;7(2):84-9.
doi: 10.4103/0974-2727.163135.

Clinicomycological Study of Dermatophytosis in South India

Lakshmi Vasantha Poluri  1 , Jyothi P Indugula  2 , Sai L Kondapaneni  1
Affiliations

Clinicomycological Study of Dermatophytosis in South India

Lakshmi Vasantha Poluri et al. J Lab Physicians. 2015 Jul-Dec.

Abstract

Introduction: Dermatophytic infections are commonly encountered a problem and constitute more than 50% of cases in dermatology outpatient departments. Diagnosis of these infections requires the proper use of laboratory methods.

Objectives: This study was conducted to know the etiology of dermatophytosis in patients attending Tertiary Care Level Hospital in South India and to compare the efficacy of Sabouraud's dextrose agar (SDA) with actidione and dermatophyte test medium (DTM) in isolating and identifying dermatophytes.

Materials and methods: A total of 110 samples which included 101 skin samples and 9 hair samples from clinically suspected dermatophytosis were collected. Direct microscopy by KOH and culture on SDA with actidione and DTM were done.

Results: Of 110 samples collected, 58.18% were KOH positive for fungal filaments and 56.36% were culture positive for dermatophytes. More number of cases were observed between age groups of 21-40 years. Males were more affected compared to females. Tinea corporis was the common clinical presentation observed (40%). Trichophyton rubrum (58.06%) was the predominant isolate recovered in all clinical presentations but Trichophyton violaceum was the most common isolate in tinea capitis. All culture positives were grown on both SDA with actidione and DTM. Appearance of growth was earlier on DTM that is, within 10 days compared to SDA with actidione where growth started appearing only after 10 days. This is statistically significant P < 0.0001 (χ(2) = 71.6). Species level identification on primary isolation was possible when grown on SDA with actidione and it was not possible with the growth on DTM on primary isolation.

Conclusion: DTM is a good screening medium in laboratory diagnosis of dermatophytosis when compared to SDA with actidione. But DTM is inferior to SDA with actidione in identification of dermatophyte species.

Keywords: Dermatophte test medium; Sabouraud's dextrose agar with actidione; dermatophytosis.

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Figures

Figure 1
Figure 1
Trichophyton rubrum. (a) Growth on Sabouraud's dextrose agar (obverse). (b) Growth on Sabouraud's dextrose agar (reverse with pigmentation). (c) Lacto phenol cotton blue mount showing tear drop shaped microconidia (×400). (d and e) Lacto phenol cotton blue mount showing pencil shaped macroconidia (×400)
Figure 2
Figure 2
Trichophyton mentagrophytes. (a) Growth on Sabouraud's dextrose agar. (b) Lacto phenol cotton blue mount showing spiral hyphae (×400). (c) Lacto phenol cotton blue mount showing macroconidia (×400). (d) Lacto phenol cotton blue mount showing Nodular organ (×400)
Figure 3
Figure 3
Epidermophyton floccosum. (a) Growth on Sabouraud's dextrose agar (obverse). (b) Growth on Sabouraud's dextrose agar (reverse). (c and d) Lacto phenol cotton blue mount showing club shaped macroconidia (×400). (e) Lacto phenol cotton blue mount showing characteristic chlamydospores (×400)
Figure 4
Figure 4
Trichophyton violaceum. (a) Growth on Sabouraud's dextrose agar (obverse with violet color pigmentation). (b) Lacto phenol cotton blue mount showing hyphae with chains of chlamydospores (×400)
Figure 5
Figure 5
Trichophyton tonsurans. (a) Growth on Sabouraud's dextrose agar (obverse). (b) Growth on Sabouraud's dextrose agar (reverse). (c) Lacto phenol cotton blue mount showing microconida (×400). (d) Lacto phenol cotton blue mount showing match stick like microconidia (×400). (e) lacto phenol cotton blue mount showing macroconidia (×400)
Figure 6
Figure 6
Trichophyton schoenleinii. Lacto phenol cotton blue mount showing antler hyphae and chlamydospores (×400)
Figure 7
Figure 7
Growth on dermatophyte test medium. (a) Trichophyton rubrum. (b) Trichophyton mentagrophytes. (c) Trichophyton tonsurans. (d) Epidermophyton floccosum. (e) Trichophyton schoenleinii. (f) Trichophyton violaceum
See this image and copyright information in PMC

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