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. 2015:2015:540916.
doi: 10.1155/2015/540916. Epub 2015 Aug 31.

Design and Formulation of Optimized Microemulsions for Dermal Delivery of Resveratrol

Vaida Juškaitė  1 , Kristina Ramanauskienė  1 , Vitalis Briedis  1
Affiliations

Design and Formulation of Optimized Microemulsions for Dermal Delivery of Resveratrol

Vaida Juškaitė et al. Evid Based Complement Alternat Med. 2015.

Abstract

The objective of this study was to formulate optimal formulations of microemulsions (MEs) and evaluate their feasibility for delivery of resveratrol into human skin ex vivo. Oil-in-water MEs were formulated using surfactant (S) PEG-8 caprylic/capric glycerides and cosurfactant (CoS) polyglyceryl-6-isostearate. Ethyl oleate was used as an oily phase. MEs were formulated using 5 : 1, 6 : 1, and 7 : 1 surfactant and cosurfactant (S : CoS) weight ratios. Pseudoternary phase diagrams were constructed and optimal compositions of MEs were obtained using Design Expert software. Mean droplet size for optimized ME formulations was determined to be 68.54 ± 1.18 nm, 66.08 ± 0.16 nm, and 66.66 ± 0.56 nm for systems with S : CoS weight ratios 5 : 1, 6 : 1, and 7 : 1, respectively. Resveratrol loading resulted in mean droplet size increase. The distribution of droplet size between fractions changed during storage of formulated MEs. Results demonstrated the increase of number of droplets and relative surface area when S : CoS weight ratios were 6 : 1 and 7 : 1 and the decrease when S : CoS weight ratio was 5 : 1. The highest penetration of resveratrol into the skin ex vivo was determined from ME with S : CoS weight ratio 5 : 1. It was demonstrated that all MEs were similar in their ability to deliver resveratrol into the skin ex vivo.

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Figures

Figure 1
Figure 1
Pseudoternary phase diagrams of ranges of stable MEs when PEG-8 caprylic/capric glycerides and polyglyceryl-6-isostearate are used at ratios 5 : 1 (a), 6 : 1, and 7 : 1 (b).
Figure 2
Figure 2
Areas of optimal composition of MEs with highest desirability parameter for compositions with ratios of PEG-8 caprylic/capric glycerides and polyglyceryl-6-isostearate, 5 : 1 (a), 6 : 1 (b), and 7 : 1 (c).
Figure 3
Figure 3
Changes in droplet size distribution in MEs during storage.
Figure 4
Figure 4
Cumulative amount of resveratrol in epidermis and dermis after application of formulations for 24 hours.
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