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. 2015 Jul;9(7):ZC61-4.
doi: 10.7860/JCDR/2015/12656.6223. Epub 2015 Jul 1.

Cd34 and Mast Cell Analysis in Normal Oral Mucosa and Different Grades of Oral Squamous Cell Carcinoma: A Comparative Study

Pargatsingh T Kathuriya  1 , Anirudha R Bartake  2 , Sangeeta J Palaskar  3 , Bindiya R Narang  2 , Swati S Patil  1 , Rasika B Pawar  1
Affiliations

Cd34 and Mast Cell Analysis in Normal Oral Mucosa and Different Grades of Oral Squamous Cell Carcinoma: A Comparative Study

Pargatsingh T Kathuriya et al. J Clin Diagn Res. 2015 Jul.

Abstract

Background: Oral Squamous Cell Carcinoma (OSCC) remains a serious health problem worldwide. Prognosis of OSCC is poor and long term survival rate still remains below 50%. Angiogenesis or neovascularisation plays an important role in tumour progression and metastasis. Mast cells have been implicated in promoting tumour angiogenesis, especially of digestive tract, little is known in OSCC.

Aim & objective: To study the correlation between blood vessel density (BVD) and mast cell density (MCD) in different grades of OSCC.

Methods: Thirty eight paraffin blocks of different grades of OSCC were retrieved from the department and sections were stained with CD34 followed by counterstaining with toluidine blue. The slides were then analysed using Leica Software (Version 4.5).

Results: Mean BVD and MCD were found to be increased in OSCC as compared to normal mucosa. Increase in BVD with co-current increase in MCD was also observed in different grades of OSCC.

Conclusion: From our study, it was concluded that, mast cells play a major role in promoting tumour angiogenesis. But, as the grade of the tumour increases, other angiogenic factors may play a more significant role than mast cells in tumour progression.

Keywords: Angiogenesis; Malignant neoplasm Progression.

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Figures

[Table/Fig-1a]:
[Table/Fig-1a]:
Pyogenic granuloma showing CD34 positive blood vessels (10X)
[Table/Fig-1b]:
[Table/Fig-1b]:
Pyogenic granuloma showing mast cells stained (purple) with Toluidine blue stain(10X)
[Table/Fig-2]:
[Table/Fig-2]:
Light microscopic view of CD34 positive blood vessels and mast cells stained by Toluidine blue in normal oral mucosa (40X) {Light yellow labels shows number of CD34 positive blood vessels in the section, Light blue labels shows number of purple coloured mast cells in the section, The approx. 40,000μm2 is the area of the given section under 40X magnification}
[Table/Fig-3]:
[Table/Fig-3]:
Light microscopic view of CD34 positive blood vessels and mast cells stained by Toluidine blue in well differentiated oral squamous cell carcinoma (40X) {Light yellow labels shows number of CD34 positive blood vessels in the section, Light blue labels shows number of purple coloured mast cells in the section, The approx. 40,000μm2 is the area of the given section under 40X magnification}
[Table/Fig-4]:
[Table/Fig-4]:
Light microscopic view of CD34 positive blood vessels and mast cells stained by Toluidine blue in moderately differentiated oral squamous cell carcinoma (40X) {Light yellow labels shows number of CD34 positive blood vessels in the section, Light blue labels shows number of purple coloured mast cells in the section, The approx. 40,000μm2 is the area of the given section under 40X magnification}
[Table/Fig-5]:
[Table/Fig-5]:
Light microscopic view of CD34 positive blood vessels and mast cells stained by Toluidine blue in poorly differentiated oral squamous cell carcinoma (40X) {Light yellow labels shows number of CD34 positive blood vessels in the section, Light blue labels shows number of purple coloured mast cells in the section, The approx. 40,000μm2 is the area of the given section under 40X magnification}
See this image and copyright information in PMC

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