Recombinant bacterial lipoproteins as vaccine candidates

Abstract

Recombinant bacterial lipoproteins (RLP) with built-in immuno-stimulating properties for novel subunit vaccine development are reviewed. This platform technology offers the following advantages: easily converts antigens into highly immunogenic RLP using a fusion sequence containing lipobox; the lipid moiety of RLP is recognized as the danger signals in the immune system through the Toll-like receptor 2, so both innate and adaptive immune responses can be induced by RLP; serves as an efficient and cost-effective bioprocess for producing RLP in Escherichia coli and the feasibility and safety of this core platform technology has been successfully demonstrated in animal model studies including meningococcal group B subunit vaccine, dengue subunit vaccine, novel subunit vaccine against Clostridium difficile-associated diseases and HPV-based immunotherapeutic vaccines.

Keywords: Braun’s lipoproteins; Clostridium difficile-associated diseases; Toll-like receptor agonist; adjuvant; cervical cancer; dengue virus; lipobox leader sequences; meningococcal group B; subunit vaccine; synthetic adjuvant PAMCSK4.