Non-structural carbohydrates in woody plants compared among laboratories

Audrey G Quentin¹, Elizabeth A Pinkard², Michael G Ryan³, David T Tissue⁴, L Scott Baggett⁵, Henry D Adams⁶, Pascale Maillard⁷, Jacqueline Marchand⁸, Simon M Landhäusser⁹, André Lacointe¹⁰, Yves Gibon¹¹, William R L Anderegg¹², Shinichi Asao¹³, Owen K Atkin¹⁴, Marc Bonhomme¹⁰, Caroline Claye¹⁵, Pak S Chow⁹, Anne Clément-Vidal¹⁶, Noel W Davies¹⁷, L Turin Dickman⁶, Rita Dumbur¹⁸, David S Ellsworth⁴, Kristen Falk¹⁹, Lucía Galiano²⁰, José M Grünzweig¹⁸, Henrik Hartmann²¹, Günter Hoch²², Sharon Hood²³, Joanna E Jones¹⁵, Takayoshi Koike²⁴, Iris Kuhlmann²¹, Francisco Lloret²⁵, Melchor Maestro²⁶, Shawn D Mansfield²⁷, Jordi Martínez-Vilalta²⁵, Mickael Maucourt²⁸, Nathan G McDowell⁶, Annick Moing¹¹, Bertrand Muller²⁹, Sergio G Nebauer³⁰, Ülo Niinemets³¹, Sara Palacio²⁶, Frida Piper³², Eran Raveh³³, Andreas Richter³⁴, Gaëlle Rolland²⁹, Teresa Rosas³⁵, Brigitte Saint Joanis¹⁰, Anna Sala²³, Renee A Smith⁴, Frank Sterck³⁶, Joseph R Stinziano³⁷, Mari Tobias³¹, Faride Unda²⁷, Makoto Watanabe³⁸, Danielle A Way³⁹, Lasantha K Weerasinghe⁴⁰, Birgit Wild⁴¹, Erin Wiley⁹, David R Woodruff⁴²

Affiliations

¹ CSIRO Land and Water, Private Bag 12, Hobart, Tasmania 7001, Australia Hawkesbury Institute for the Environment, University of Western Sydney, Richmond, NSW 2753, Australia audrey.quentin@csiro.au.
² CSIRO Land and Water, Private Bag 12, Hobart, Tasmania 7001, Australia.
³ Natural Resources Ecology Laboratory, Colorado State University, Fort Collins, CO 80523-1499, USA Graduate Degree Program in Ecology, Colorado State University, Fort Collins, CO 80523-1401, USA USDA Forest Service, Rocky Mountain Research Station, Fort Collins, CO 80521, USA.
⁴ Hawkesbury Institute for the Environment, University of Western Sydney, Richmond, NSW 2753, Australia.
⁵ USDA Forest Service, Rocky Mountain Research Station, Fort Collins, CO 80521, USA.
⁶ Earth and Environmental Sciences Division, Los Alamos National Laboratory, Los Alamos, NM, 87545, USA.
⁷ INRA, UMR 1137, Ecologie et Ecophysiologie Forestières, Centre de Nancy, F-54280 Champenoux, France.
⁸ INRA, UMR 1137, Ecologie et Ecophysiologie Forestières, Plateforme Technique d'Ecologie Fonctionnelle (OC 081) Centre de Nancy, F-54280 Champenoux, France.
⁹ Department of Renewable Resources, University of Alberta, Edmonton, AB, T6G 2E3, Canada.
¹⁰ INRA, UMR 0547 PIAF, F:63100 Clermont-Ferrand, France Clermont Université, Université Blaise Pascal, UMR 0547 PIAF, F:6310 Clermont-Ferrand, France.
¹¹ UMR1332, Biologie du Fruit et Pathologie, INRA, Bordeaux University, 71 avenue Edouard Bourlaux, F-33140 Villenave d'Ornon, France Plateforme Métabolome du Centre de Génomique Fonctionnelle Bordeaux, MetaboHUB, IBVM, Centre INRA, 71 avenue Edouard Bourlaux, F-33140 Villenave d'Ornon, France.
¹² Princeton Environmental Institute, Princeton University, Princeton NJ 08540, USA.
¹³ Natural Resources Ecology Laboratory, Colorado State University, Fort Collins, CO 80523-1499, USA Graduate Degree Program in Ecology, Colorado State University, Fort Collins, CO 80523-1401, USA.
¹⁴ Division of Plant Sciences, Research School of Biology, Building 46, The Australian National University, Canberra, ACT, 2601, Australia ARC Centre of Excellence in Plant Energy Biology, The Australian National University, Canberra, ACT, 2601, Australia.
¹⁵ Tasmanian Institute of Agriculture, School of Land and Food, Private Bag 98, University of Tasmania, Hobart, Tasmania 7001, Australia.
¹⁶ CIRAD, UMR AGAP, F-34398 Montpellier, France.
¹⁷ Central Science Laboratory, Private Bag 74, University of Tasmania, Hobart, Tasmania 7001, Australia.
¹⁸ Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 7610001, Israel.
¹⁹ Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR 97331, USA.
²⁰ Swiss Federal Research Institute WSL, CH-8903 Birmensdorf, Switzerland Institute of Hydrology, Freiburg University, Fahnenbergplatz, D-79098 Freiburg, Germany.
²¹ Max Planck Institute for Biogeochemistry, Hans-Knöll Str. 10, 07745 Jena, Germany.
²² Department of Environmental Sciences - Botany, University of Basel, Schönbeinstrasse 6, CH-4056 Basel, Switzerland.
²³ Division of Biological Sciences, University of Montana, Missoula MT-59812, USA.
²⁴ Silviculture and Forest Ecological Studies, Hokkaido University Sapporo, Hokkaido 060-8589, Japan.
²⁵ CREAF, Cerdanyola del Vallès E-08193 Barcelona, Spain Universidad Autònoma Barcelona, Cerdanyola del Vallès E-08193 Barcelona, Spain.
²⁶ Instituto Pirenaico de Ecología (IPE-CSIC), Av. Nuestra Señora de la Victoria s/n, 22700 Jaca, Huesca, Spain.
²⁷ Department of Wood Science, University of British Columbia, V6T 1Z4 Vancouver, Canada.
²⁸ Plateforme Métabolome du Centre de Génomique Fonctionnelle Bordeaux, MetaboHUB, IBVM, Centre INRA, 71 avenue Edouard Bourlaux, F-33140 Villenave d'Ornon, France Université Bordeaux, UMR 1332, Biologie du Fruit et Pathologie, 71 avenue Edouard Bourlaux, F-33140 Villenave d'Ornon, France.
²⁹ INRA, UMR 759 LEPSE, Montpellier, F-34060 France.
³⁰ Plant Production Department, Universitat Politécnica de Valéncia, Camino de vera s.n. 46022-Valencia, Spain.
³¹ Department of Plant Physiology, Estonian University of Life Sciences, Kreutzwaldi 1, 51014 Tartu, Estonia.
³² Centro de Investigación en Ecosistemas de la Patagonia (CIEP), Simpson 471, Coyhaique, Chile.
³³ Department of Fruit Trees Sciences, Institute of Plant Sciences, A.R.O., Gilat Research Center, D.N. Negev 85289, Israel.
³⁴ Department of Microbiology and Ecosystem Science, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
³⁵ CREAF, Cerdanyola del Vallès E-08193 Barcelona, Spain.
³⁶ Forest Ecology and Forest Management Group, Wageningen University, Postbox 47, 6700 AA, Wageningen, the Netherlands.
³⁷ Department of Biology, Western University, 1151 Richmond Street, London, N6A 5B7, ON, Canada.
³⁸ Institute of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo 183-8509, Japan.
³⁹ Department of Biology, Western University, 1151 Richmond Street, London, N6A 5B7, ON, Canada Nicholas School of the Environment, Duke University, Box 90328, Durham, NC 27708, USA.
⁴⁰ Division of Plant Sciences, Research School of Biology, Building 46, The Australian National University, Canberra, ACT, 2601, Australia Faculty of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka.
⁴¹ Department of Microbiology and Ecosystem Science, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria Department of Earth Sciences, University of Gothenburg, Guldhedsgatan 5A, 40530 Gothenburg, Sweden.
⁴² USDA Forest Service, Forestry Sciences Laboratory, Corvallis, OR 97331, USA.

PMID: 26423132
DOI: 10.1093/treephys/tpv073

Comparative Study

Non-structural carbohydrates in woody plants compared among laboratories

Audrey G Quentin et al. Tree Physiol. 2015 Nov.

. 2015 Nov;35(11):1146-65.

doi: 10.1093/treephys/tpv073. Epub 2015 Sep 29.

Authors

Affiliations

¹ CSIRO Land and Water, Private Bag 12, Hobart, Tasmania 7001, Australia Hawkesbury Institute for the Environment, University of Western Sydney, Richmond, NSW 2753, Australia audrey.quentin@csiro.au.
² CSIRO Land and Water, Private Bag 12, Hobart, Tasmania 7001, Australia.
³ Natural Resources Ecology Laboratory, Colorado State University, Fort Collins, CO 80523-1499, USA Graduate Degree Program in Ecology, Colorado State University, Fort Collins, CO 80523-1401, USA USDA Forest Service, Rocky Mountain Research Station, Fort Collins, CO 80521, USA.
⁴ Hawkesbury Institute for the Environment, University of Western Sydney, Richmond, NSW 2753, Australia.
⁵ USDA Forest Service, Rocky Mountain Research Station, Fort Collins, CO 80521, USA.
⁶ Earth and Environmental Sciences Division, Los Alamos National Laboratory, Los Alamos, NM, 87545, USA.
⁷ INRA, UMR 1137, Ecologie et Ecophysiologie Forestières, Centre de Nancy, F-54280 Champenoux, France.
⁸ INRA, UMR 1137, Ecologie et Ecophysiologie Forestières, Plateforme Technique d'Ecologie Fonctionnelle (OC 081) Centre de Nancy, F-54280 Champenoux, France.
⁹ Department of Renewable Resources, University of Alberta, Edmonton, AB, T6G 2E3, Canada.
¹⁰ INRA, UMR 0547 PIAF, F:63100 Clermont-Ferrand, France Clermont Université, Université Blaise Pascal, UMR 0547 PIAF, F:6310 Clermont-Ferrand, France.
¹¹ UMR1332, Biologie du Fruit et Pathologie, INRA, Bordeaux University, 71 avenue Edouard Bourlaux, F-33140 Villenave d'Ornon, France Plateforme Métabolome du Centre de Génomique Fonctionnelle Bordeaux, MetaboHUB, IBVM, Centre INRA, 71 avenue Edouard Bourlaux, F-33140 Villenave d'Ornon, France.
¹² Princeton Environmental Institute, Princeton University, Princeton NJ 08540, USA.
¹³ Natural Resources Ecology Laboratory, Colorado State University, Fort Collins, CO 80523-1499, USA Graduate Degree Program in Ecology, Colorado State University, Fort Collins, CO 80523-1401, USA.
¹⁴ Division of Plant Sciences, Research School of Biology, Building 46, The Australian National University, Canberra, ACT, 2601, Australia ARC Centre of Excellence in Plant Energy Biology, The Australian National University, Canberra, ACT, 2601, Australia.
¹⁵ Tasmanian Institute of Agriculture, School of Land and Food, Private Bag 98, University of Tasmania, Hobart, Tasmania 7001, Australia.
¹⁶ CIRAD, UMR AGAP, F-34398 Montpellier, France.
¹⁷ Central Science Laboratory, Private Bag 74, University of Tasmania, Hobart, Tasmania 7001, Australia.
¹⁸ Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 7610001, Israel.
¹⁹ Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR 97331, USA.
²⁰ Swiss Federal Research Institute WSL, CH-8903 Birmensdorf, Switzerland Institute of Hydrology, Freiburg University, Fahnenbergplatz, D-79098 Freiburg, Germany.
²¹ Max Planck Institute for Biogeochemistry, Hans-Knöll Str. 10, 07745 Jena, Germany.
²² Department of Environmental Sciences - Botany, University of Basel, Schönbeinstrasse 6, CH-4056 Basel, Switzerland.
²³ Division of Biological Sciences, University of Montana, Missoula MT-59812, USA.
²⁴ Silviculture and Forest Ecological Studies, Hokkaido University Sapporo, Hokkaido 060-8589, Japan.
²⁵ CREAF, Cerdanyola del Vallès E-08193 Barcelona, Spain Universidad Autònoma Barcelona, Cerdanyola del Vallès E-08193 Barcelona, Spain.
²⁶ Instituto Pirenaico de Ecología (IPE-CSIC), Av. Nuestra Señora de la Victoria s/n, 22700 Jaca, Huesca, Spain.
²⁷ Department of Wood Science, University of British Columbia, V6T 1Z4 Vancouver, Canada.
²⁸ Plateforme Métabolome du Centre de Génomique Fonctionnelle Bordeaux, MetaboHUB, IBVM, Centre INRA, 71 avenue Edouard Bourlaux, F-33140 Villenave d'Ornon, France Université Bordeaux, UMR 1332, Biologie du Fruit et Pathologie, 71 avenue Edouard Bourlaux, F-33140 Villenave d'Ornon, France.
²⁹ INRA, UMR 759 LEPSE, Montpellier, F-34060 France.
³⁰ Plant Production Department, Universitat Politécnica de Valéncia, Camino de vera s.n. 46022-Valencia, Spain.
³¹ Department of Plant Physiology, Estonian University of Life Sciences, Kreutzwaldi 1, 51014 Tartu, Estonia.
³² Centro de Investigación en Ecosistemas de la Patagonia (CIEP), Simpson 471, Coyhaique, Chile.
³³ Department of Fruit Trees Sciences, Institute of Plant Sciences, A.R.O., Gilat Research Center, D.N. Negev 85289, Israel.
³⁴ Department of Microbiology and Ecosystem Science, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
³⁵ CREAF, Cerdanyola del Vallès E-08193 Barcelona, Spain.
³⁶ Forest Ecology and Forest Management Group, Wageningen University, Postbox 47, 6700 AA, Wageningen, the Netherlands.
³⁷ Department of Biology, Western University, 1151 Richmond Street, London, N6A 5B7, ON, Canada.
³⁸ Institute of Agriculture, Tokyo University of Agriculture and Technology Fuchu, Tokyo 183-8509, Japan.
³⁹ Department of Biology, Western University, 1151 Richmond Street, London, N6A 5B7, ON, Canada Nicholas School of the Environment, Duke University, Box 90328, Durham, NC 27708, USA.
⁴⁰ Division of Plant Sciences, Research School of Biology, Building 46, The Australian National University, Canberra, ACT, 2601, Australia Faculty of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka.
⁴¹ Department of Microbiology and Ecosystem Science, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria Department of Earth Sciences, University of Gothenburg, Guldhedsgatan 5A, 40530 Gothenburg, Sweden.
⁴² USDA Forest Service, Forestry Sciences Laboratory, Corvallis, OR 97331, USA.

PMID: 26423132
DOI: 10.1093/treephys/tpv073

Abstract

Non-structural carbohydrates (NSC) in plant tissue are frequently quantified to make inferences about plant responses to environmental conditions. Laboratories publishing estimates of NSC of woody plants use many different methods to evaluate NSC. We asked whether NSC estimates in the recent literature could be quantitatively compared among studies. We also asked whether any differences among laboratories were related to the extraction and quantification methods used to determine starch and sugar concentrations. These questions were addressed by sending sub-samples collected from five woody plant tissues, which varied in NSC content and chemical composition, to 29 laboratories. Each laboratory analyzed the samples with their laboratory-specific protocols, based on recent publications, to determine concentrations of soluble sugars, starch and their sum, total NSC. Laboratory estimates differed substantially for all samples. For example, estimates for Eucalyptus globulus leaves (EGL) varied from 23 to 116 (mean = 56) mg g(-1) for soluble sugars, 6-533 (mean = 94) mg g(-1) for starch and 53-649 (mean = 153) mg g(-1) for total NSC. Mixed model analysis of variance showed that much of the variability among laboratories was unrelated to the categories we used for extraction and quantification methods (method category R(2) = 0.05-0.12 for soluble sugars, 0.10-0.33 for starch and 0.01-0.09 for total NSC). For EGL, the difference between the highest and lowest least squares means for categories in the mixed model analysis was 33 mg g(-1) for total NSC, compared with the range of laboratory estimates of 596 mg g(-1). Laboratories were reasonably consistent in their ranks of estimates among tissues for starch (r = 0.41-0.91), but less so for total NSC (r = 0.45-0.84) and soluble sugars (r = 0.11-0.83). Our results show that NSC estimates for woody plant tissues cannot be compared among laboratories. The relative changes in NSC between treatments measured within a laboratory may be comparable within and between laboratories, especially for starch. To obtain comparable NSC estimates, we suggest that users can either adopt the reference method given in this publication, or report estimates for a portion of samples using the reference method, and report estimates for a standard reference material. Researchers interested in NSC estimates should work to identify and adopt standard methods.

Keywords: extraction and quantification consistency; non-structural carbohydrate chemical analysis; particle size; reference method; soluble sugars; standardization; starch.

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A carbohydrate quandary.
Germino MJ. Germino MJ. Tree Physiol. 2015 Nov;35(11):1141-5. doi: 10.1093/treephys/tpv109. Epub 2015 Oct 27. Tree Physiol. 2015. PMID: 26507272 No abstract available.

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Non-structural carbohydrates in woody plants compared among laboratories

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Non-structural carbohydrates in woody plants compared among laboratories

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