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. 2015 Dec;76(12):923-7.
doi: 10.1016/j.humimm.2015.09.015. Epub 2015 Sep 28.

Advances in DNA sequencing technologies for high resolution HLA typing

Nezih Cereb  1 , Hwa Ran Kim  2 , Jaejun Ryu  2 , Soo Young Yang  2
Affiliations

Advances in DNA sequencing technologies for high resolution HLA typing

Nezih Cereb et al. Hum Immunol. 2015 Dec.

Abstract

This communication describes our experience in large-scale G group-level high resolution HLA typing using three different DNA sequencing platforms - ABI 3730 xl, Illumina MiSeq and PacBio RS II. Recent advances in DNA sequencing technologies, so-called next generation sequencing (NGS), have brought breakthroughs in deciphering the genetic information in all living species at a large scale and at an affordable level. The NGS DNA indexing system allows sequencing multiple genes for large number of individuals in a single run. Our laboratory has adopted and used these technologies for HLA molecular testing services. We found that each sequencing technology has its own strengths and weaknesses, and their sequencing performances complement each other. HLA genes are highly complex and genotyping them is quite challenging. Using these three sequencing platforms, we were able to meet all requirements for G group-level high resolution and high volume HLA typing.

Keywords: DNA sequence-based HLA typing (SBT); HLA typing; MiSeq; NGS; New alleles; PacBio; Sanger sequencing.

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