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. 2015:1327:59-74.
doi: 10.1007/978-1-4939-2842-2_6.

Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System

John A Calarco  1 , Ari E Friedland  2
Affiliations

Creating Genome Modifications in C. elegans Using the CRISPR/Cas9 System

John A Calarco et al. Methods Mol Biol. 2015.

Abstract

The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe a method using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective approach for making heritable changes to the worm genome.

Keywords: C. elegans; CRISPR; Cas9; Genome editing; Genome engineering.

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