Corynebacterium diphtheriae in a free-roaming red fox: case report and historical review on diphtheria in animals

Abstract

Objectives: Corynebacterium diphtheriae, the classical causative agent of diphtheria, is considered to be nearly restricted to humans. Here we report the first finding of a non-toxigenic C. diphtheriae biovar belfanti strain in a free-roaming wild animal.

Methods: The strain obtained from the subcutis and mammary gland of a dead red fox (Vulpes vulpes) was characterized by biochemical and molecular methods including MALDI-TOF and Multi Locus Sequence Typing. Since C. diphtheriae infections of animals, usually with close contact to humans, are reported only very rarely, an intense review comprising also scientific literature from the beginning of the 20th century was performed.

Results: Besides the present case, only 11 previously reported C. diphtheriae animal infections could be verified using current scientific criteria.

Conclusions: Our report is the first on the isolation of C. diphtheriae from a wildlife animal without any previous human contact. In contrast, the very few unambiguous publications on C. diphtheriae in animals referred to livestock or pet animals with close human contact. C. diphtheriae carriage in animals has to be considered as an exceptionally rare event.

Keywords: Animals; Corynebacterium diphtheriae; Diphtheria; Historical review; Wildlife; Zoonosis.