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. 2015:1329:57-65.
doi: 10.1007/978-1-4939-2871-2_5.

The Role of a Destabilized Membrane for OMP Insertion

Ashlee M Plummer  1 , Dennis Gessmann  1 , Karen G Fleming  2
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The Role of a Destabilized Membrane for OMP Insertion

Ashlee M Plummer et al. Methods Mol Biol. 2015.

Erratum in

Abstract

Here we describe the procedures used in our laboratory for the in vitro investigation of the apparent folding kinetics as well as the folding efficiencies of outer membrane proteins (OMPs). Because microbial OMPs display a change in their gel migration upon folding, the usage of traditional gel electrophoresis is a standard method of folding analysis. Additional aspects of the method we detail herein include the preparation and storage of OMP stocks, the setup procedures for a folding reaction, and the analysis of fraction folded from scanned gel images.

Keywords: BamA; Extrusion; Kinetics; Large unilamellar vesicles; Membrane protein folding; OMP.

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