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. 2015 Nov:118:173-5.
doi: 10.1016/j.mimet.2015.09.019. Epub 2015 Oct 1.

Establishment and validation of a 384-well antibacterial assay amenable for high-throughput screening and combination testing

Arti Mishra  1 , Svetlana V Dobritsa  2 , Marie-Laure Crouch  3 , John Rabenstein  3 , Joycelyn Xiang Yi Lee  1 , Saravanakumar Dhakshinamoorthy  4
Affiliations

Establishment and validation of a 384-well antibacterial assay amenable for high-throughput screening and combination testing

Arti Mishra et al. J Microbiol Methods. 2015 Nov.

Abstract

A 384-well-based antibacterial assay amenable for high-throughput screening and combination testing is described. The assay uses 100-500nL of test compounds and tolerates up to 2.5% dimethyl sulfoxide concentrations. It can be used for screening compound libraries and testing combinatory/synergistic/antagonistic effects of antibiotics, small molecules, and natural product extracts.

Keywords: Antibacterial assay; Combination testing; DMSO tolerance; HTS.

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