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. 2016 Jan 4:211:58-63.
doi: 10.1016/j.virusres.2015.09.022. Epub 2015 Oct 3.

Electrophoretic RNA genomic profiles of Brazilian Picobirnavirus (PBV) strains and molecular characterization of a PBV isolated from diarrheic calf

Elisabete Takiuchi  1 , Rubia Macedo  2 , Andressa Fernanda Kunz  2 , Jessica Cristhine Gallego  2 , Janaina Lustosa de Mello  2 , Rodrigo Alejandro Arellano Otonel  3 , Amauri Alcindo Alfieri  3
Affiliations

Electrophoretic RNA genomic profiles of Brazilian Picobirnavirus (PBV) strains and molecular characterization of a PBV isolated from diarrheic calf

Elisabete Takiuchi et al. Virus Res. .

Abstract

Picobirnavirus (PBV) belongs to the family Picobirnaviridae. PBV are a group of emerging non-enveloped viruses, with a bisegmented double-stranded RNA genome that can infect a wide range of hosts. This study reports the occurrence of PBV in fecal samples from five Brazilian dairy cattle herds. From the 289 stool samples of individual calves analyzed by silver-stained polyacrylamide gel electrophoresis (ss-PAGE) the PBV was detected in 8.3 % (24/289), of which 10.2% (18/176) had diarrheic consistency. Of the 24 positive samples in ss-PAGE, 5 (20.8%) of them showed a small electrophoretic profile and 19 (79.2%) samples had large profile. From the 24 positives samples by ss-PAGE, 15 (62.5%) were successfully amplified (201 bp) using GI specific primers targeting the RdRp gene of PBV. The analysis of nucleotide identity matrix revealed that the bovine PBV strain identified in this study, showed the highest nucleotide identity (81%) with PBV strain detected in turkey (MD-2010/HM803965). This is the first nucleotide sequence of a bovine PBV strain in the American continent and the first detection of small genome profile of PBV-like strains in bovine hosts.

Keywords: Bovine; PAGE; Phylogeny; Picobirnavirus; RT-PCR.

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Figures

Fig. 1
Fig. 1
Picobirnavirus (PBV) electrophoretic genomic profiles detected in dairy calves fecal samples by silver stained-polyacrylamide gel electrophoresis (ss-PAGE). (Panel A). Lanes 1: Genome pattern of specie A bovine rotavirus; lane 2: negative sample; lane 3: large genome profile of PBV. (Panel B). Lane 1: Genome pattern of specie A bovine rotavirus; lanes 2 and 3: negative samples; lane 4: small genome profile of PBV.
Fig. 2
Fig. 2
Phylogenetic analysis among Picobirnavirus (PBV) genogroup I based on the partial fragment of the RdRp gene (segment 2). The bovine PBV sequence obtained in this study is indicated with the symbol “♦”. The phylogenetic tree was constructed by using the Neighbor-Joining method and the Kimura 2-parameter nucletotide substitution. Bootstrapping was statistically supported with 1000 replicates. Scale bar indicates nucleotide substitutions per site.
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References

    1. Alfieri A.F., Alfieri A.A., Resende J.S., Resende M. A new bi-segmented double stranded RNA virus in avian feces. Arq. Bras. Med. Vet. Zootec. 1989;40:437–440.
    1. Alfieri A.A., Parazzi M.E., Takiuchi E., Médici K.C., Alfieri A.F. Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998–2002. Trop. Anim. Health Prod. 2006;38:521–526. - PMC - PubMed
    1. Bhattacharya R., Sahoo G.C., Nayak M.K., Saha D.R., Sur D., Naik T.N., Bhattacharya S.K., Krishnan T. Molecular epidemiology of human picobirnaviruses among children of a slum community in Kolkata, India. Infect. Genet. Evol. 2006;6:453–458. - PubMed
    1. Bhattacharya S., Sahoo G.C., Nayak M.K., Rajendran K., Dutta P., Mitra U., Bhattacharya M.K., Naik T.N., Bhattacharya S.K., Krishnan T. Detection of Genogroup I and II human picobirnaviruses showing small genomic RNA profile causing acute watery diarrhoea among children in Kolkata, India. Infect. Genet. Evol. 2007;7(2):229–238. - PubMed
    1. Boom R., Sol C.J., Salimans M.M., Jansen C.L., Wertheim-van Dillen P.M., van der Noordaa J. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 1990;28:495–503. - PMC - PubMed