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. 1989 Jan;27(1):82-7.
doi: 10.1128/jcm.27.1.82-87.1989.

Isolation and characterization of Klebsiella pneumoniae unencapsulated mutants

V J Benedí  1 , B Ciurana, J M Tomás
Affiliations

Isolation and characterization of Klebsiella pneumoniae unencapsulated mutants

V J Benedí et al. J Clin Microbiol. 1989 Jan.

Abstract

Klebsiella pneumoniae mutants were obtained after UV irradiation and negative selection with anticapsular serum. Unencapsulation, rather than expression of a structurally altered capsule, was found in the mutants. The mutant strains showed no alterations in their outer membrane proteins and lipopolysaccharide, and a great similarity with the wild type in the properties tested (serum resistance, antimicrobial sensitivity, and lipopolysaccharide-specific bacteriophage sensitivity), with the exception of a higher cell surface hydrophobicity and resistance to bacteriophage FC3-9.

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