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. 2015 Oct 5;10(10):e0139666.
doi: 10.1371/journal.pone.0139666. eCollection 2015.

Construction of a Genetic Linkage Map and Identification of QTLs for Seed Weight and Seed Size Traits in Lentil (Lens culinaris Medik.)

Priyanka Verma  1 , Richa Goyal  2 , R K Chahota  3 , Tilak R Sharma  3 , M Z Abdin  4 , Sabhyata Bhatia  2
Affiliations

Construction of a Genetic Linkage Map and Identification of QTLs for Seed Weight and Seed Size Traits in Lentil (Lens culinaris Medik.)

Priyanka Verma et al. PLoS One. .

Abstract

Seed weight and seed size both are quantitative traits and have been considered as important components of grain yield, thus identification of quantitative trait loci (QTL) for seed traits in lentil (Lens culinaris) would be beneficial for the improvement of grain yield. Hence the main objective of this study was to identify QTLs for seed traits using an intraspecific mapping population derived from a cross between L. culinaris cv. Precoz (seed weight-5.1g, seed size-5.7mm) and L. culinaris cv. L830 (seed weight-2.2g, seed size-4mm) comprising 126 F8-RILs. For this, two microsatellite genomic libraries enriched for (GA/CT) and (GAA/CTT) motif were constructed which resulted in the development of 501 new genomic SSR markers. Six hundred forty seven SSR markers (including 146 previously published) were screened for parental polymorphism and 219 (33.8%) were found to be polymorphic among the parents. Of these 216 were mapped on seven linkage groups at LOD4.0 spanning 1183.7cM with an average marker density of 5.48cM. Phenotypic data from the RILs was used to identify QTLs for the seed weight and seed size traits by single marker analysis (SMA) followed by composite interval mapping (CIM) which resulted in one QTL each for the 2 traits (qSW and qSS) that were co-localized on LG4 and explained 48.4% and 27.5% of phenotypic variance respectively. The current study would serve as a strong foundation for further validation and fine mapping for utilization in lentil breeding programs.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. An intraspecific linkage map of L. culinaris based on RIL mapping population generated by crossing L. culinaris cv. Precoz× L. culinaris cv. L830.
The map was generated with 219 polymorphic genomic SSR markers using JoinMap version 4.0 at a LOD value of 4.0, with Kosambi mapping function. A total of 216 markers were mapped on seven linkage groups (LGs) (LG1 to LG7). The previously mapped markers used in this study are underlined. The distorted markers have been marked with “*” on the right side of the marker name.
Fig 2
Fig 2. Frequency distribution of A. SW (seed weight in g) and B. SS (seed size in mm) phenotypic traits for the 126 RILs as depicted by the histogram.
The phenotype of both the parents P1 (L. culinaris cv. Precoz) and P2 (L. culinaris cv. L830) are indicated by arrows.
Fig 3
Fig 3. Chromosomal locations of detected QTL.
LOD peak value of ≥2.8 was considered to indicate a significant QTL interval. The QTLs are indicated by rectangular bars of different shades in the right side of the linkage groups followed by the name of the QTL. The LOD score plot of individual trait [(A) seed weight and (B) seed size] is displayed on the right side of the bars locating the marker positions. The region of identified QTLs on the linkage group is highlighted by yellow color.
See this image and copyright information in PMC

References

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