Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Mar;25(3):231-2.
doi: 10.1111/exd.12872. Epub 2016 Jan 15.

Classical and non-classical metabolic transformation of vitamin D in dermal fibroblasts

Andrzej T Slominski  1   2 , Tae-Kang Kim  1 , Wei Li  3 , Robert C Tuckey  4
Affiliations

Classical and non-classical metabolic transformation of vitamin D in dermal fibroblasts

Andrzej T Slominski et al. Exp Dermatol. 2016 Mar.
No abstract available

Keywords: 20-hydroxyvitamin D3; CYP11A1; metabolism; skin.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Dose dependent production of hydroxyvitamin D3 metabolites in fibroblasts. Fibroblasts were incubated without D3 (A, D), or with 50 μM (B, E) or 500 μM (C, F) D3. Extracts of samples were directly analysed by LC-MS using a Xevo™ G2-S qTOF tandem mass spectrometer with m/z = 383.3 [M+H-H2O]+ for mono-hydroxyvitamin D3 and m/z = 399.3 [M+H-H2O]+ for di-hydroxyvitamins D3.
Figure 2
Figure 2
Identification of mono- and dihydroxy-vitamin D3 metabolites in fibroblasts incubated with 500 μM D3. Hydroxyderivatives in extracts were pre-purified by HPLC on a 250 mm C18 column with an acetonitrile gradient then analysed by UPC LC-QTOF MS. The LC-MS spectra show extracted masses corresponding to ions at m/z = 401.3 [M+H]+ for 22(OH)D3 and 25(OH)D3; 383.3 [M+H-H2O]+ for 20(OH)D3; 417.3 [M+H]+ for 1,20(OH)2D3; 399.3 [M+H-H2O]+ for 1,25(OH)2D3, 20,22(OH)2D3 and 20,23(OH)2D3. Inserts show the mass spectra at [M+H]+ and [M+H-H2O]+ recorded for each identified species at the corresponding RT.
See this image and copyright information in PMC

References

    1. Bikle DD. Vitamin D: an ancient hormone. Experimental Dermatology. 2011;20(1):7–13. - PubMed
    1. Holick MF. Vitamin D: A millenium perspective. J Cell Biochem. 2003;88(2):296–307. - PubMed
    1. Guryev O, et al. A pathway for the metabolism of vitamin D3: unique hydroxylated metabolites formed during catalysis with cytochrome P450scc (CYP11A1). Proc Natl Acad Sci U S A. 2003;100(25):14754–9. - PMC - PubMed
    1. Slominski A, et al. The cytochrome P450scc system opens an alternate pathway of vitamin D3 metabolism. FEBS J. 2005;272(16):4080–90. - PMC - PubMed
    1. Tuckey RC, et al. Pathways and products for the metabolism of vitamin D3 by cytochrome P450scc. FEBS J. 2008;275(10):2585–96. - PMC - PubMed

Publication types