Better growth of Campylobacter jejuni using simple Fortner's principle & candle extinction jar

Abstract

C. jejuni isolated from patients of childhood diarrhoea and chicken intestines were examined for better growth in both liquid and solid media under three different environments of candle extinction jar with or without an Escherichia coli inoculated plate and three such plates in a sealed jar. Better growth by one log in liquid media was obtained at 24 and 48 h with bacterial counts of about ca. 10(7) and ca. 10(9) respectively, and larger colony size at 24 h on solid medium by inclusion of a single Esch. coli inoculated plate following Fortner's principle rather than candle jar alone. Inclusion of three Esch. coli plates yielded similar results at 48 h but lesser growth at 24 h and a decline at 72 h. Most of the C. jejuni strains failed to adapt to grow in presence of air.