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. 2015:1339:85-99.
doi: 10.1007/978-1-4939-2929-0_5.

Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root

M Jesús Andrés-Manzano  1 , Vicente Andrés  2 , Beatriz Dorado  1
Affiliations

Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root

M Jesús Andrés-Manzano et al. Methods Mol Biol. 2015.

Abstract

Methods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide. These stains can provide quantitative data about the impact of a genetic or environmental factor on atherosclerotic burden and on the initiation, progression, or regression of the disease, and can also be used to evaluate the efficacy of drugs designed to prevent or treat atherosclerosis. This chapter provides protocols for quantifying atherosclerotic burden in mouse aorta and aortic root, including methods for dissection, Oil Red O staining, hematoxylin-eosin staining, and image analysis.

Keywords: Aorta; Aortic root; Atherosclerosis; Dissection; Hematoxylin-eosin; Oil Red O.

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